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目的研究惊厥患儿血清中胶原纤维酸性蛋白(Glial fibrillary acidic protein,GFAP)及髓鞘碱性蛋白(Myelin basic protein,MBP)的表达水平,以探讨血清中GFAP及MBP的水平与惊厥患儿脑损伤之间的关系及血清中GFAP及MBP表达水平的临床意义。方法选取2014年7月—2015年7月在唐山市妇幼保健院神经内科住院的惊厥患儿116例作为观察组;根据惊厥病因分为热性惊厥组、病毒性脑炎组、癫组;根据惊厥发作频率及持续时间分为惊厥组1和惊厥组2。选取同期住院的非惊厥患儿42例作为对照组,采用酶联免疫吸附测定法(Enzyme-linked immuno sorbent assay,ELISA)测定患儿血清中GFAP及MBP的水平,并分别比较血清中GFAP及MBP在观察组及对照组中表达的差异。结果观察组惊厥患儿血清中GFAP及MBP的表达水平显著高于对照组(P<0.01);而热性惊厥组、病毒性脑炎组、癫组之间血清中GFAP及MBP的表达水平差异无统计学意义(P>0.05);惊厥组2患儿血清中GFAP及MBP的表达水平显著高于惊厥组1(P<0.01)。结论惊厥性相关疾病可使患儿血清中GFAP及MBP的表达水平升高,但惊厥所致脑损伤程度与惊厥性相关疾病病种无关,而与惊厥发作频率和持续时间有关,惊厥发作频率越频繁,持续时间越长,患儿血清中GFAP及MBP的表达水平越高,造成的脑损伤越严重,GFAP及MBP可作为惊厥所致脑损伤临床检测生化指标。
Objective To investigate the serum levels of glial fibrillary acidic protein (GFAP) and myelin basic protein (MBP) in sera of children with convulsions and to explore the relationship between serum levels of GFAP and MBP, The relationship between the injury and serum GFAP and MBP expression of clinical significance. Methods Seventy-six convulsions hospitalized in Department of Neurology, Tangshan MCH Hospital from July 2014 to July 2015 were selected as the observation group. According to the etiology of seizures, they were divided into febrile seizure group, viral encephalitis group and epilepsy group. According to the frequency and duration of seizures were divided into seizure group 1 and seizure group 2. Forty-two non-convulsive children hospitalized in the same period were selected as the control group, serum GFAP and MBP levels were measured by Enzyme-linked immuno sorbent assay (ELISA), and serum GFAP and MBP Differences in expression between the observation group and the control group. Results The serum levels of GFAP and MBP in children with seizures in the observation group were significantly higher than those in the control group (P <0.01). However, the levels of GFAP and MBP in sera from febrile seizures, viral encephalitis and epilepsy groups There was no significant difference between the two groups (P> 0.05). The levels of GFAP and MBP in sera of 2 children with convulsion group were significantly higher than those of convulsion group 1 (P <0.01). CONCLUSIONS: Seizure-related diseases can increase the expression of GFAP and MBP in sera of children, but the degree of brain injury induced by seizures has nothing to do with the seizure-related diseases, but with the frequency and duration of seizures, the frequency of convulsions Frequent, longer duration, the higher serum GFAP and MBP expression levels in children, resulting in more serious brain damage, GFAP and MBP can be used as a clinical test biochemical indicators of brain injury caused by convulsions.