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以三种栽培大麦品种为母本,三种球茎大麦为父本进行杂交。诱导栽培大麦单倍体,结果杂交成胚率平均为47.9％,幼胚离体培养成苗率平均为54.0％。这些胚培苗的根尖细胞染色体数目分别含有7,14,21条,不同组合杂交后代含有的染色体数目不同,所有含有7条染色体的后代,其染色体C-带带型显示为父本栽培大麦的染色体,表明该后代为栽培大麦单倍体,用滴加0.04％秋水仙素的Knop溶液进行单倍体植株的染色体加倍,结果加倍率达90％以上,另外,我们还讨论了染色体消失法诱导单倍体的优越性及其机理。
Three kinds of cultivated barley varieties as the female parent, three kinds of bulb barley as the male parent. Induction of barley haploid cultivars, the results of the embryo formation rate of hybridization average of 47.9%, immature embryos cultured in vitro rate was 54.0%. The chromosome numbers of root tip cells in these embryos were respectively 7, 14, and 21, and the number of chromosomes in different combinations of hybrid progeny was different. All the progeny with 7 chromosomes showed the chromosome C-banding pattern as male-cultivated barley The results showed that the offspring was haploid barley cultivars. Chromosome haploid plants were doubled with Knop solution with 0.04% colchicine, and the doubling rate was over 90%. In addition, we also discussed the chromosomal ablation method The Superiority and Mechanism of Induction Haploid.