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目的通过对出生后不同时期大鼠趾长伸肌(EDL)和比目鱼肌(SOL)运动神经元(Mn)群树突发育的研究,了解脊髓Mn嫌Golgi树突在发育过程中的变化以及这两种不同功能特性Mn群所表现的差异,从而推断其功能解剖学意义。方法以霍乱毒素B亚单位结合辣根过氧化物酶(CB-HRP)对EDL-Mn和SOL-Mn进行逆行追踪,显示两者的树突。结果以张力性活动方式为主的SOL-Mn群生后一直具有丰富的嫌Golgi树突,以位相性活动方式为主的EDL-Mn群,在生后早期也具有较丰富的嫌Golgi树突,但在成年后却明显减少,其面密度值仅是前者的一半。结论脊髓Mn嫌Golgi树突与Mn的活动方式密切相关,嫌Golgi树突参与调节脊髓Mn活动的“旁侧回路”,特别是对张力性慢肌Mn的调控。以上结果为Mn的“双重回路调控”即旁侧回路及核心回路假说进一步提供功能解剖学依据。
OBJECTIVE: To study the dendritic development of the motor neuron (Mn) population of EDL and SOL in rats at different stages after birth, The differences between the two different functional properties of Mn population inferred their functional anatomic significance. Methods The retrograde tracing of EDL-Mn and SOL-Mn using cholera toxin B subunit combined with horseradish peroxidase (CB-HRP) showed dendritic dendritic and dendritic splicing. Results The results showed that the SOL-Mn group, which is mainly composed of tension activity, had a rich background of Golgi dendrites. The EDL-Mn group dominated by the phase activity mode also had abundant Golgi dendrites in the early postnatal period, But decreased significantly after adulthood, with the areal density of only half of the former. Conclusions The Golgi dendrites in the spinal cord are closely related to the activity pattern of Mn. It is suspected that the Golgi dendrites participate in the regulation of the “side loop” of Mn activity in the spinal cord, especially the regulation of Mn in tension. The above results provide a functional anatomical basis for the “double loop regulation” of Mn, ie, the side loop and core loop hypothesis.