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为探讨苜蓿与无芒雀麦混播草地苜蓿越冬率及抗寒效应,选用龙牧806、公农1号、WL319HQ和敖汉4个苜蓿品种与草地羊茅同行3∶7混播,分析整个越冬期间单、混播根系形态学指标及生理生化指标动态变化情况,并通过隶属函数评价各混播组合的抗寒性能。结果表明,苜蓿与无芒雀麦混播处理越冬率均高于其单播处理;随着外界环境低温的胁迫,同一苜蓿品种混播处理根颈直径、主根直径及侧根数均高于单播处理,但根颈入土深度、主根长度,单播处理高于混播处理;越冬前随自然温度的下降,苜蓿根颈直径呈现先增加后降低趋势,11月15日—4月30日整个越冬期根颈直径变化不明显;抗寒性高的品种根颈分枝较多。4个苜蓿品种单播及与无芒雀麦混播苜蓿根系可溶性糖、可溶性蛋白及游离脯氨酸均随着气温下降其含量增加,翌年春随着气温回升而降低,其中可溶性糖、可溶性蛋白及游离脯氨酸含量在11月中旬达到最大值;过氧化物酶(POD)则是随着温度变化在整个越冬期呈现先升后降再上升的变化趋势。通过越冬率调查和运用隶属函数法进行抗寒性综合评判,得出供试材料抗寒性大小依次为公农1号+无芒雀麦>公农1号>WL319HQ+无芒雀麦>WL319HQ>龙牧806+无芒雀麦>龙牧806>敖汉+无芒雀麦>敖汉。
In order to explore the overwintering rate and cold resistance of alfalfa mixed with meadow grass, we selected 3 alfalfa varieties of Longmu 806, Gongnong 1, WL319HQ and Aohan for 3: 7 mixed sowing, and analyzed the whole winter period Single and mixed sowing root morphology and physiological and biochemical indicators of dynamic changes, and through the membership function evaluation of mixed-sowing combination of the cold resistance. The results showed that the overwintering rates of alfalfa and Bromus inermis were higher than those of unicceted treatment. With the external environment of low temperature stress, root diameter, root diameter and lateral root number of the same alfalfa variety were higher than the unicasting treatment, However, root-in-soil depth, main root length and unicomponent treatment were higher than those of mixed-crop treatment. With the decrease of natural temperature before winter, the diameter of root neck of alfalfa first increased and then decreased. From November 15 to April 30, Diameter change is not obvious; cold hardiness varieties of root neck more branches. The contents of soluble sugar, soluble protein and free proline in four alfalfa cultivars and the mixed-sowed Bromus mandshurica increased with the temperature decreasing, and the spring of next year decreased with the temperature rising. The content of soluble sugar, soluble protein and The content of free proline peaked in mid-November. Peroxidase (POD) showed the trend of increasing first, then decreasing, then increasing again with the change of temperature. Through the overwintering rate survey and applying the membership function method to evaluate the cold resistance, the results showed that the cold resistance of the tested materials was in the order of: Gongnong 1 + Brome grass> Gongnong 1> WL319HQ + Bromegrass> WL319HQ> Longmu 806 + Broccoli> Longmu 806> Aohan + Brome grassless> Aohan.