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目的:比较15个产地人参中4种氧化还原酶的活力差异。方法:采用中性磷酸缓冲溶液提取粗酶液。应用紫外分光光度法分别测定过氧化物酶(POD)、过氧化氢酶(CAT)、多酚氧化酶(PPO)和抗坏血酸过氧化物酶(APX)的活力。结果:POD活力以吉林省敦化青钩子乡和吉林省安图万宝镇人参活力较高,吉林省抚松黄泥村人参活力最低;CAT活力以安图万宝镇人参活力最高,黑龙江省穆棱市人参活力最低;PPO活力以安图万宝镇人参活力最高,黑龙江省牡丹江市人参活力最低;APX活力以吉林省通化市通化县人参活力最高,抚松板石河村人参活力最低。15个不同产地样品可聚为5类。结论:POD、CAT、PPO、APX活力可以作为人参种源区域划分的内在指标。
Objective: To compare the differences of vitality of four redox enzymes in 15 ginsengs. Methods: Neutral phosphate buffer solution was used to extract crude enzyme solution. The activities of peroxidase (POD), catalase (CAT), polyphenol oxidase (PPO) and ascorbate peroxidase (APX) were determined by UV spectrophotometry. Results: The activity of POD was as high as that of Qingtouzi Township, Dunhua County, Jilin Province and Wanbao Township, Antu County, Jilin Province. The activity of ginseng was the lowest in Huangni Village, Fusong, Jilin Province. The activity of CAT was the highest in Antu Wanbao Town. Muling, Heilongjiang Province The vitality of ginseng was the lowest in PPO vitality, the lowest in ginseng in Mudanjiang City, Heilongjiang Province. The vitality of ginseng in APX vitality was the highest in Tonghua County, Tonghua County, Jilin Province. 15 samples from different areas can be grouped into five categories. CONCLUSION: The activities of POD, CAT, PPO and APX can be used as intrinsic indexes for the regionalization of provenances in ginseng.