啮齿类动物胰岛分离的应用广泛 ,其方法也早已建立 ,但影响胰岛分离的因素较多 ,使获得足量、纯净、存活和功能良好的胰岛常较困难 ,且胰岛分离的稳定性和一致性亦难令人满意。因此 ,针对分离技术、纯化方法、分离剂、消化酶及消化条件等诸方面进行了广泛的改进 ,胰岛的收获量、纯度及活力等均有显著的提高。 Rodent islets are widely used and methods have been established, but there are many factors that affect islet isolation, making it difficult to obtain adequate, pure, viable and well-characterized islets, and the stability and consistency of islet isolation Is also difficult to satisfactory. Therefore, a wide range of improvements have been made in terms of separation techniques, purification methods, separating agents, digestive enzymes and digestion conditions, and the amount of islets harvested, purity and vigor have been significantly improved.