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目的:以新鲜蘘荷Zingiber mioga花蕾为原料,探讨蘘荷花蕾不同提取部位的抗氧化作用,并筛选其有效抑菌部位。方法:蘘荷花蕾用甲醇提取浓缩,依次萃取得到石油醚、二氯甲烷、乙酸乙酯、正丁醇和水等5个萃取部位后,采用1,1-二苯基-2-三硝基苯肼(DPPH)自由基清除模型评价不同萃取部位抗氧化能力,用打孔扩散法考察萃取部位对大肠埃希菌、沙门氏菌、金黄色葡萄球菌和铜绿假单胞菌的体外抑菌活性。结果:加热回流法提取蘘荷花蕾,提取率为3.1%,其中乙酸乙酯部位DPPH自由基清除能力最高,半数抑制率(IC50)达到(26.22±1.12)mg·L-1,对铜绿假单胞菌的抑菌圈达到(10±1)mm,石油醚部位抑菌活性最高,对金黄色葡萄球菌的最低抑菌浓度(MIC)最低,达到25.0 mg·L-1。结论:蘘荷花蕾各部位均有一定的抗氧化能力,综合抑菌效果比较石油醚部位>二氯甲烷部位>乙酸乙酯部位>甲醇提取物,为蘘荷花蕾抗氧化产品、抑菌剂的开发利用提供理论基础。
OBJECTIVE: To study the anti-oxidation effect of different extracts of Lotus flower buds using Zingiber mioga flower buds as raw material, and to screen the effective antibacterial sites. Methods: The lotus buds were extracted with methanol, concentrated and extracted successively with five extraction fractions of petroleum ether, dichloromethane, ethyl acetate, n-butanol and water. Then 1,1-diphenyl-2-trinitrobenzene Hydrazine (DPPH) free radical scavenging model was used to evaluate the anti-oxidative ability of different extraction sites. The antibacterial activity of the extraction sites on Escherichia coli, Salmonella, Staphylococcus aureus and Pseudomonas aeruginosa were investigated by using the perforated diffusion method. Results: The flower buds were extracted by heating and refluxing method. The extraction rate was 3.1%. The DPPH radical scavenging activity of ethyl acetate was the highest and the IC50 was (26.22 ± 1.12) mg · L-1. Bacteriostatic zone reached (10 ± 1) mm. The antibacterial activity of petroleum ether was the highest. The minimum inhibitory concentration (MIC) of Staphylococcus aureus was 25.0 mg · L-1. CONCLUSION: All the buds of lotus flower have certain anti-oxidative abilities. Compared with petroleum ether fraction> dichloromethane fraction> ethyl acetate fraction> methanol extract, Provide a theoretical basis for development and utilization.