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目的研究小鼠初级听皮层SOM+神经元接受刺激后突触输入的时空特性。方法通过局部场电位记录方法对初级听皮层进行定位后,采用电压钳全细胞记录方法在离体脑片上分别记录SOM+神经元和锥体神经元在接受刺激后诱发的兴奋后突触后电流和抑制性突触后电流。通过对两类神经元的兴奋性突触后电流和抑制性突触后电流时间特性以及幅度上的比较,从而分析SOM+神经元突触输入的时空特性。结果 SOM+神经元与锥体神经元的突触输入在时间特性上表现出相似的起始潜伏期。SOM+神经元的兴奋性突触后电流和抑制性突触后电流的峰值潜伏期[(14.35±2.74)ms,(18.26±3.24)ms]明显要短于锥体神经元的兴奋性突触后电流和抑制性突触后电流的峰值潜伏期[(18.81±3.76)ms,(21.08±3.93)ms],差异具有统计学意义(EPSC P<0.05,IPSC P<0.01)。在突触后电流的幅度上两类神经元差异没有统计学意义(P>0.05)。结论 SOM+中间神经元接受的突触输入与锥体神经元接受的突触输入在基本电生理特性上类似,但时间特性上的差异提示两者接受的突触输入在皮层内环路来源上可能存在一定差异。
Objective To study the temporal and spatial characteristics of synaptic input after stimulating SOM + neurons in primary auditory cortex of mice. Methods After localization of primary auditory cortex by local field potential recording, voltage-clamp whole-cell recording was used to record the post-stimulation postsynaptic currents of SOM + neurons and pyramidal neurons induced by stimulation in vitro Inhibitory postsynaptic currents. The spatial and temporal characteristics of SOM + neuronal synaptic input were analyzed by comparing the time characteristics and amplitudes of excitatory postsynaptic currents and inhibitory postsynaptic currents of the two types of neurons. Results The synaptic input of SOM + neurons and pyramidal neurons showed a similar initial latency in temporal characteristics. The peak latency of excitatory postsynaptic currents and inhibitory postsynaptic currents of SOM + neurons [(14.35 ± 2.74) ms, (18.26 ± 3.24) ms] was significantly shorter than that of the pyramidal neurons And peak latency of inhibitory postsynaptic currents [(18.81 ± 3.76) ms, (21.08 ± 3.93) ms] respectively. The difference was statistically significant (EPSC P <0.05, IPSC P <0.01). There was no significant difference in the magnitude of postsynaptic currents between the two types of neurons (P> 0.05). Conclusions The synaptic inputs received by SOM + interneurons are similar to those received by pyramidal neurons in basic electrophysiological characteristics, but differences in temporal characteristics suggest that both the accepted synaptic inputs may be at the origin of the cortical inner loop There are some differences.