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活体SCE试验中的主要试剂BudR可用国产的IudR代替。方法是把IudR按1.25～1.50/kg体重埋入动物皮下组织,让动物自然吸收,井参与细胞DNA的合成,从而显示出染色体二单体深浅不同的差别染色。在理入IudR的同时,将一定量的诱变物注射入动物体内,然后取骨髓细胞制片,可观察到染色体的SCE频率明显升高,用此法检测了甲基磺酸甲酯、黄曲霉毒素B_1、痢特灵、灭虫灵等4个化学物,均获得满意结果。从而得出IudR应用于活体SCE试验中使方法更加简便、经济和易行,是检测环境诱变物的一个好方法的结论。
The main reagent, BudR, in live SCE assays can be replaced with homemade IudR. The method is to bury IudR in subcutaneous tissues of animals at 1.25~1.50/kg body weight to allow the animals to naturally absorb them. Wells participate in the synthesis of cellular DNA, and thus show different differential staining of chromosome 2 monomers. At the same time as IudR was ligated, a certain amount of mutagen was injected into the animal, and then the bone marrow cells were taken for production. The chromosome SCE frequency was significantly increased. Methyl methanesulfonate and yellow were detected by this method. Four chemicals, such as Aspergillus B_1, Furazolidone, and Diflubenzuron, all received satisfactory results. Therefore, it is concluded that the application of IudR in living SCE assays is simpler, more economical and feasible, and is a good method for detecting environmental mutagens.