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利用SSR分子标记,对2 0世纪5 0年代我国引入海岛棉以来培育的4 5个国内品种(系)及8个国外品种的遗传多样性进行研究。通过2 5 6对SSR引物的筛选,选择2 4对扩增效果好的引物对5 3个海岛棉种质资源进行遗传多样性的检测分析,共检测出10 6个等位位点,每对引物等位位点数在2～8之间,平均为4 4。其中多态性等位基因变异97个,占91 5 %。位点多态性信息含量平均为0 6 88,最高为0 84 8,最低为0 2 4 5。利用NTSYSpc2 1软件,分别计算农艺经济性状的欧氏距离(Euclid)和分子标记数据的Jaccard系数矩阵,采用UPGMA法对所选材料进行聚类分析。结果表明,两个树状聚类图基本吻合,5 3个品种被分为两大类,与系谱来源一致。实验证明SSR分子标记在鉴别品种和品种遗传多样性研究方面具有重要作用。
Using SSR markers, the genetic diversity of 45 domestic breeds (lines) and 8 foreign breeds cultivated since the introduction of island cotton in China in the 1950s was studied. A total of 10 6 alleles were detected by PCR-SSR primer selection and 24 pairs of primer pairs with good amplification. Genetic diversity of 53 island cotton germplasm resources were detected and analyzed. The number of alleles in the primer ranged from 2 to 8 with an average of 4 4. Among them, 97 alleles were polymorphic, accounting for 91.5%. The average polymorphism information content of loci was 0 6 88, the highest was 0 84 8 and the lowest was 0 2 4 5. Using NTSYSpc2 1 software, the Euclid distance of agronomic characters and the Jaccard coefficient matrix of molecular marker data were calculated respectively. The UPGMA method was used to cluster the selected materials. The results showed that the two dendrogram clustering basically accordant, 53 varieties were divided into two categories, consistent with pedigree source. Experiments show that SSR molecular markers play an important role in the identification of genetic diversity of cultivars and cultivars.