固相萃取-超高效液相色谱-串联质谱法测定畜产品中残留的22种磺胺类药物

来源 :中国食品卫生杂志 | 被引量 : 0次 | 上传用户:qgdjyahaha
下载到本地 , 更方便阅读
声明 : 本文档内容版权归属内容提供方 , 如果您对本文有版权争议 , 可与客服联系进行内容授权或下架
论文部分内容阅读
目的 建立畜产品中22种磺胺类药物残留的超高效液相色谱-串联质谱(UPLC-MS/MS)测定方法.方法 样品经乙腈涡旋提取,正己烷除脂后,阳离子交换固相萃取柱净化,再经Waters Acquity UPLC BEH C18(2.1 mm×100 mm,1.7μm)色谱柱分离,在电喷雾正离子多反应监测模式下进行测定.结果 22种磺胺类药物在一定的浓度范围内线性关系良好,相关系数(r) >0.997.在猪肉、猪肝、猪肾3种基质中,22种磺胺类药物的检出限为0.03~0.3 μg/kg,定量限为0.1~1μg/kg.在1、2、5μg/kg三个添加水平下,其加标回收率为63.0%~ 115.6%,相对标准偏差为1.2% ~15.9%.结论 该方法快速、简便、准确可靠,适用于畜产品中22种磺胺类药物的同时检测.“,”Objective To establish a method for the determination of 22 sulfonamides in livestock products by ultra high performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS).Methods Samples were extracted by acetonitrile and the fat was removed by hexane.Then the extract was purified by cation exchange solid phase extraction.The analytes were separated on a Waters Acquity UPLC BEH C18 column (2.1 mm× 100 mm,1.7 μm) and detected by positive electrospray ionization in multiple reactions monitoring (MRM).Results The method showed a good linearity within a certain concentration ranges with relative coefficient r > 0.997.The detection limits of 22 sulfonamides in porcine muscle,liver,and kidney were 0.03-0.3 μg/kg,and the quantification limits were 0.1-1 μg/kg.The average recoveries of 22 sulfonamides at the spiking levels of 1,2 and 5 μg/kg were in the range of 63.0%-115.6%,with the relative standard deviations in the range of 1.2%-15.9%.Conclusion The method was rapid,simple and accurate for the simultaneous determination of 22 sulfonamides in livestock products.
其他文献
The tooth belongs to the trigeminal sensory pathway.Dental damage has been associated with impairments in the central nervous system that may be mediated by inj
2014年7月18日,英国国立健康与临床优化研究所( NICE)正式发布心血管疾病( CVD)一级和二级预防调脂和心血管危险评估指南。与2008年版NICE指南相比,本次更新的指南提倡对患者进行更
Propofol and remifentanil alter intracellular Ca2+ concentration ([Ca2+]i) in neural stem/progen-itor cells by activating γ-aminobutyric acid type A receptors
This study analyzed 85 articles indexed by the Web of Science concerning nerve regeneration and osseoperception during tooth loss repair.Using the Web of Scienc
目的 概率评估上海市非伤寒沙门菌(以下简称“沙门菌”)感染的流行病学负担,为上海市食源性疾病的防控提供决策依据.方法 随机抽取上海市60个街道/乡镇,通过入户问卷调查获得