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目的 建立畜产品中22种磺胺类药物残留的超高效液相色谱-串联质谱(UPLC-MS/MS)测定方法.方法 样品经乙腈涡旋提取,正己烷除脂后,阳离子交换固相萃取柱净化,再经Waters Acquity UPLC BEH C18(2.1 mm×100 mm,1.7μm)色谱柱分离,在电喷雾正离子多反应监测模式下进行测定.结果 22种磺胺类药物在一定的浓度范围内线性关系良好,相关系数(r) ＞0.997.在猪肉、猪肝、猪肾3种基质中,22种磺胺类药物的检出限为0.03～0.3 μg/kg,定量限为0.1～1μg/kg.在1、2、5μg/kg三个添加水平下,其加标回收率为63.0％～ 115.6％,相对标准偏差为1.2％ ～15.9％.结论 该方法快速、简便、准确可靠,适用于畜产品中22种磺胺类药物的同时检测.“,”Objective To establish a method for the determination of 22 sulfonamides in livestock products by ultra high performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS).Methods Samples were extracted by acetonitrile and the fat was removed by hexane.Then the extract was purified by cation exchange solid phase extraction.The analytes were separated on a Waters Acquity UPLC BEH C18 column (2.1 mm× 100 mm,1.7 μm) and detected by positive electrospray ionization in multiple reactions monitoring (MRM).Results The method showed a good linearity within a certain concentration ranges with relative coefficient r ＞ 0.997.The detection limits of 22 sulfonamides in porcine muscle,liver,and kidney were 0.03-0.3 μg/kg,and the quantification limits were 0.1-1 μg/kg.The average recoveries of 22 sulfonamides at the spiking levels of 1,2 and 5 μg/kg were in the range of 63.0％-115.6％,with the relative standard deviations in the range of 1.2％-15.9％.Conclusion The method was rapid,simple and accurate for the simultaneous determination of 22 sulfonamides in livestock products.