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目的建立小鼠流感病毒性肺炎湿热证模型,为中药应用于湿热证治疗提供模型基础。方法将小鼠随机分为正常组、病毒组(单纯病毒感染造模)、模型组(肥甘饮食+湿热环境+病毒感染)。采用反转录-聚合酶链反应(RT-PCR)检测小鼠肺病毒含量。并与正常组、病毒组小鼠进行肛温、血脂、肺病变程度等方面的比较。结果正常组、病毒组、模型组小鼠的体重分别为(21.5±0.91),(19.44±1.55),(22.16±0.94)g,模型组高于正常组、病毒组,差异有统计学意义(P<0.05);小鼠肛温分别为(35.8±0.4),(35.6±0.3),(36.6±0.4)℃,模型组温度增高(P<0.05)。此外模型组血脂亦较其他2组升高明显。模型组、病毒组的肺指数和肺病变程度和正常组比较差异有统计学意义(P<0.05)。结论该模型无论发病条件、病变程度,还有主要症状体征均近似于中医湿热证型,操作简单,重复性好,有较高的应用价值。
Objective To establish a mouse model of influenza virus pneumonia damp-heat syndrome and provide a model basis for the application of traditional Chinese medicine in the treatment of damp-heat syndrome. Methods The mice were randomly divided into normal group, virus group (model of virus infection alone), model group (fat diet + hot and humid environment + virus infection). Mouse lung virus content was detected by reverse transcription-polymerase chain reaction (RT-PCR). And with the normal group, the virus group mice rectal temperature, blood lipids, lung lesions and other aspects of the comparison. Results The body weight of the normal group, the virus group and the model group were (21.5 ± 0.91), (19.44 ± 1.55) and (22.16 ± 0.94) g, respectively. The model group was higher than the normal group and the virus group (P <0.05). The rectal temperature in mice was (35.8 ± 0.4), (35.6 ± 0.3) and (36.6 ± 0.4) ℃, respectively. The temperature of model group was higher than that of model group (P <0.05). In addition, the model group of blood lipids than the other two groups increased significantly. The pulmonary index and pulmonary lesion in model group and virus group were significantly different from those in normal group (P <0.05). Conclusion The model is similar to damp-heat syndrome of TCM regardless of the condition of onset, degree of lesion and main symptoms and signs. It has simple operation, good repeatability and high application value.