为了研究B7 1分子功能性mAb (6H2 )对表达B7 1分子的B系淋巴瘤Raji细胞的生物效应。分别运用免疫荧光标记和流式细胞仪技术 (FACS )、台盼蓝拒染试验、MTT试验和RT PCR半定量分析 ,分析 6H2对Raji细胞的生长抑制和凋亡诱导作用。结果显示 ,6H2能抑制天然表达B7 1分子的人B系淋巴瘤Raji细胞的生长 ,产生细胞周期G2 /M的阻滞 ,上调细胞表面CD18和CD95 (Fas )的表达 ,且能使Raji细胞中Bax的相对转录表达显著增加 ,从而诱导Raji细胞的凋亡。 6H2为功能性抗人B7 1分子特异性mAb ,具有抑制Raji细胞生长和凋亡诱导作用 ,在B系淋巴瘤研究和生物治疗中具有应用价值。 In order to investigate the biological effects of functional B7 mAb (6H2) on B lymphoma Raji cells expressing B7 molecule. Immunofluorescence labeling and flow cytometry (FACS), trypan blue exclusion assay, MTT assay and RT PCR semi-quantitative analysis were used to detect the growth inhibition and apoptosis induction of Raji cells by 6H2. The results showed that 6H2 could inhibit the growth of human B lymphoma Raji cells, which naturally express B7 1 molecules, block the cell cycle G2 / M, upregulate the expression of CD18 and CD95 (Fas) on the cell surface, Bax relative transcriptional expression was significantly increased, thereby inducing Raji cell apoptosis. 6H2 is a functional anti-human B7 1 molecule-specific mAb, which can inhibit the growth of Raji cells and induce apoptosis. It has application value in the study of B lymphoma and biological therapy.