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为筛选异黑成熟颗粒中甘草、地锦草、铁线蕨和香青兰最佳的醇提取工艺,采用单因素试验,以甘草酸含量和醇浸出物量为指标,对影响提取效果的乙醇浓度A、提取时间B、乙醇用量C和提取次数D进行考查,确定提取次数为2次,选择乙醇浓度为55%、65%、75%,乙醇用量为10、12、14倍量,提取时间为1.0、1.5、2.0h。进一步采用L9(34)正交试验设计优选甘草、地锦草、铁线蕨和香青兰醇提取条件,对实验各关键因素变化范围内均衡抽样,使每次试验都具有较强的代表性。结果表明,乙醇浓度对提取效果有显著影响,而提取时间、乙醇用量对提取效果无显著性影响,最佳提取工艺为A2B2C3。鉴于乙醇用量和提取时间对提取效果无显著性影响,为降低成本、节省工时,将B2改为B1,将C3改为C1,由此得提取工艺为A2B1C1。确定药材醇提取条件:65%乙醇10倍量,提取2次,每次1h。由此,经过正交试验法优选的提取工艺设计合理,结果稳定可行,符合大工业生产要求。上述实验结果可为异黑成熟颗粒制备工艺的确定提供实验依据。
In order to screen the best alcohol extraction technology of Glycyrrhiza uralensis, Elymus radix, Cyanophyta and Cymbidium hybridum, the single factor experiment was used to measure the contents of ethanol extract A , The extraction time B, the amount of ethanol C and the number of extraction D were examined to determine the number of extraction was 2 times, select the ethanol concentration of 55%, 65%, 75%, the amount of ethanol was 10,12,14 times the amount of extraction time of 1.0 , 1.5,2.0h. The orthogonal design of L9 (34) was also adopted to optimize the extraction conditions of licorice root, radix alcynae, fern and strychnine, and balanced sampling was carried out in the variation range of each key factor of experiment, which made each experiment have strong representativeness. The results showed that the concentration of ethanol had a significant effect on the extraction efficiency, while the extraction time and the amount of ethanol had no significant effect on the extraction efficiency. The optimum extraction process was A2B2C3. In view of the amount of ethanol and extraction time on the extraction effect was no significant impact, in order to reduce costs and save time, the B2 to B1, C3 to C1, which was A2B1C1 extraction process. Determine the medicinal alcohol extraction conditions: 65% ethanol 10 times the amount of extraction 2 times, each time 1h. As a result, the optimal extraction process through orthogonal test method is reasonable and the result is stable and feasible, which meets the requirements of large-scale industrial production. The above experimental results can provide experimental evidence for the determination of the preparation process of different black mature particles.