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目的探讨节气与松果腺-褪黑素的相关性。方法 96只Wistar大鼠分别于春分、秋分、夏至、冬至前40天随机分为生理组、手术组和伪手术组,每组各8只。手术组于春分、秋分、夏至、冬至(简称二分、二至)前1个月制备松果腺动物摘除模型,伪手术组只进行手术操作但不摘除松果腺,3组大鼠在相同条件下饲养到二分、二至当日20:00后取材检测四季血清和肺组织褪黑素(Mel)含量。结果生理组大鼠血清Mel春分显著高于其他3个季节(P<0.01);夏至低于秋分和冬至(P<0.05或P<0.01)。肺组织Mel春分显著高于其他3个季节(P<0.01);冬至高于夏至(P<0.05)。血清Mel春分、夏至生理组高于伪手术组(P<0.05);夏至手术组高于伪手术组(P<0.05)。肺组织Mel春分伪手术组显著高于生理组、手术组(P<0.01)。结论全年生理状态下大鼠血清和肺组织Mel呈现出春分最高,夏至最低的季节变化特点。松果腺作为重要中介物质,参与中医“肺应秋”的调控机制。
Objective To explore the correlation between solar term and pineal gland melatonin. Methods Ninety-six Wistar rats were randomly divided into physiological group, operation group and sham operation group, with 8 rats in each group. The pineal gland removal model was prepared one month prior to the vernal equinox, autumn equinox, the summer solstice, and the winter solstice (shortened two to two) in the surgery group. Only the operation was performed in the sham group but the pineal gland was not removed. Under the same conditions Under the feeding to two points, two to the day after 20:00 material seasons sera and lung tissue melatonin (Mel) content. Results Compared with the other three seasons (P <0.01), the mean serum Mel score of rats in physiology group was lower than that of autumn and winter solstice (P <0.05 or P <0.01). The score of Mel in lung tissue was significantly higher than that in the other three seasons (P <0.01), higher in winter solstice than in summer solstice (P <0.05). Serum Mel vernal equinox was higher in the summer solstice than in the sham-operated group (P <0.05), and higher in the summer solstice group than in the sham-operated group (P <0.05). The Mel group was significantly higher than that of the physiology group and operation group (P <0.01). Conclusion In the whole year physiological state of rat serum and lung tissue Mel showed the highest vernal equinox and the lowest seasonal change in summer solstice. Pineal gland as an important intermediary substance, involved in Chinese medicine “lung should fall” regulatory mechanism.