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目的 :探讨镍铬合金表面蛋白膜对合金抗微生物腐蚀能力的影响。方法 :镍铬合金试件分别用纯人工唾液(AS)(设为对照组)、含1%小牛血清蛋白的人工唾液(BSA)和含0.22%溶菌酶的人工唾液(LSZ)(分别为实验组)进行浸泡处理,再置于变形链球菌菌液中分别培养12、24、36、48 h后取出,检测电化学阻抗谱(EIS)及动态极化曲线(POT),了解镍铬合金表面腐蚀特性。采用SPSS17.0软件包对数据进行统计学分析。结果:电化学阻抗谱显示,12 h时,实验组抗腐蚀性能强于对照组,且LSZ组强于BSA组(P<0.05);24 h时,实验组间无显著差异(P>0.05),但强于对照组(P<0.05);36 h时,LSZ组抗腐蚀性能最弱,AS组与BSA组无显著性差异(P>0.05);48 h时,对照组抗腐蚀性能强于实验组,且LSZ组强于BSA组(P<0.05)。动态极化曲线结果与电化学阻抗谱基本一致。结论:当镍铬合金表面黏附有BSA或LSZ时,早期可有效抑制变形链球菌的腐蚀作用,且LSZ有更好的抑制效果。随着细菌的持续作用,当表面蛋白膜被逐渐消耗后,镍铬合金对抗变形链球菌的腐蚀能力则会较未经蛋白膜黏附的试件更弱,即合金表面在蛋白膜被去除后较初始状态抗微生物腐蚀性能有所下降,且BSA的作用更明显。
Objective: To investigate the effect of nickel-chromium alloy surface protein film on the antimicrobial corrosion resistance of alloy. Methods: Ni-Cr alloy specimens were treated with pure artificial saliva (AS) (control group), artificial saliva (BSA) containing 1% bovine serum albumin and artificial saliva (LSZ) containing 0.22% lysozyme Experimental group) were immersed, and then placed in Streptococcus mutans culture were cultured for 12,24,36,48 h after removal, detection of electrochemical impedance spectroscopy (EIS) and dynamic polarization curve (POT), understand the nickel-chromium alloy Surface corrosion characteristics. SPSS17.0 software package for statistical analysis of the data. Results: The electrochemical impedance spectroscopy showed that the corrosion resistance of the experimental group was stronger than that of the control group at 12 h and that of the LSZ group was stronger than that of the BSA group (P <0.05). There was no significant difference between the experimental groups at 24 h (P> 0.05) , But stronger than the control group (P <0.05). At 36 h, the corrosion resistance of LSZ group was the weakest, but there was no significant difference between AS group and BSA group (P> 0.05) Experimental group, and LSZ group was stronger than BSA group (P <0.05). The result of dynamic polarization curve is basically the same with that of electrochemical impedance spectroscopy. Conclusion: When BSA or LSZ adheres to the surface of Ni-Cr alloy, the corrosion of Streptococcus mutans can be effectively inhibited in the early stage and LSZ has a better inhibitory effect. With the continued role of bacteria, when the surface protein membrane is gradually consumed, the corrosion resistance of nickel-chromium alloy against Streptococcus mutans will be weaker than that of the sample without adhesion of protein membrane, that is, the surface of the alloy after the protein membrane is removed The initial state of antimicrobial corrosion decreased, and the role of BSA more obvious.