探讨了黄芩中的黄酮类化合物千层纸黄素A对人宫颈癌细胞HeLa S3的作用。 方法:用MEM+10% FCS的培养基培养HeLa S3细胞,接种于96孔微板(3 000个/孔),添加受试药物后第3d用荧光试剂alamar蓝检测细胞增殖抑制作用,并计算IC50。用琼脂糖凝胶电泳法分析DNA片段;Hoechst 33342染色 The effects of Flavonoid Astragaloside A in Astragalus membranaceus on HeLa S3 cells were studied. Methods: HeLa S3 cells were cultured in MEM+10% FCS medium and plated in 96-well microplates (3 000 cells/well). After the addition of test drugs, the inhibitory effect of cell proliferation was measured on the 3rd day after the addition of the fluorescent reagent alamar blue. IC50. Analysis of DNA fragments by agarose gel electrophoresis; Hoechst 33342 staining