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本研究用未经初乳喂养的新生猪模拟人类弯曲菌病,建立了一种实用的动物模型。7只未经初乳喂养的新生猪经8h禁食后,口饲空肠弯曲菌(CJ)24h培养物(1×10~8～7×10~8cfu/ml),每只40ml,另3只口饲大肠杆菌作对照。接种后,每天观察并记录小猪发病的临床表现。粪便标本接种于3种不同培养基:(1)Tergitol 7琼脂;(2)含5%枸橼酸盐牛血和壮观霉素的胰胨(400μg/ml)大豆琼脂;(3)含4%枸橼酸盐牛血的Butzler培养基。分别作沙门氏菌、Serpulina hyodysenteria和弯曲菌培养。小猪于接种后第3、4和6天处死,其小肠、结肠、盲肠及其他器官作病理和电镜检查。
In this study, non-colostrum-fed newborn pigs were used to simulate Campylobacter spp., And a practical animal model was established. Seven non-colostrum-fed newborn pigs were fed with Campylobacter jejuni (CJ) for 24h culture (1 × 10 ~ 8 ~ 7 × 10 ~ 8cfu / ml) Escherichia coli as a control. After inoculation, observe and record the clinical manifestations of piglets every day. Stool specimens were inoculated into 3 different media: (1) Tergitol 7 agar; (2) soy agar with 5% citrate bovine blood and spectinomycin (400 g / ml); Butzler medium with citrate bovine blood. Salmonella, Serpulina hyodysenteria and Campylobacter were respectively cultured. Piglets were sacrificed on days 3, 4 and 6 after inoculation and their small intestine, colon, cecum and other organs were examined for pathology and electron microscopy.