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目的 观察在热带风暴条件下落海１８ 小时后的船员血清酶含量（或活性）、钙磷浓度、ＴＮＦα、ＩＬ－２及ＩＬ－１２（Ｐ７０）含量的变化。方法 对３１ 名落水受海水浸泡的船员于入院后第１天、第８天的血清、尿液、外周血样本进行检测（称船员组），另取３４ 名体检健康者的样本作为对照检测（称对照组）。血清ＣｕＺｎ－ＳＯＤ、ＴＮＦα及ＩＬ－２ 含量测定用ＲＩＡ法；ＣＫ、ＣＫ－ＭＢ、ＬＤＨ及α－ＨＢＤＨ 活性测定用ＣＯＢＡＳＦＡＲＡ Ⅱ全自动生化分析仪；血清及尿液中钙、磷浓度测定也用前述分析仪；血清ＩＬ－１２（Ｐ７０ ）含量检测用ＥＬＩＳＡ法；常规计数外周血白细胞。结果 船员组入院第１ 天、第８ 天血清ＣｕＺｎ－ＳＯＤ含量及钙、磷浓度显著低于对照组，尿液钙、磷浓度改变与其血清相似。船员组入院第１ 天血清ＣＫ、ＣＫ－ＭＢ及α－ＨＢ－ＤＨ 活性显著高于对照组，第８天值与对照组值相近；两组ＬＤＨ活性无显著性差异；船员组入院第１ 天、第８ 天ＴＮＦα含量显著高于对照组，而ＩＬ－２ 含量显著低于对照组；船员组入院第１ 天ＩＬ－１２（Ｐ７０）含量显著低于对照组而第８天恢复。第１天白细胞数显著高于对照组，而第８ 天与对照组相近。结论 热带风暴条件下受海?
Objective To observe the changes of serum enzyme content (or activity), calcium and phosphorus concentration, TNFα, IL-2 and IL-12 (P70) levels of seafarers 18 hours after sea-going in the tropical storm. Methods Seventy-one sea-water-immersed crew members were tested on the first day and the eighth day after admission by the seafarer, urine and peripheral blood samples, and another 34 healthy subjects were taken as the control test Said control group). Serum CuZn-SOD, TNFα and IL-2 levels were measured by RIA method; CK, CK-MB, LDH and α-HBDH activity was measured by COBASFARA Ⅱ automatic biochemical analyzer; serum and urine calcium and phosphorus concentrations were also used The aforementioned analyzer; serum IL-12 (P70) content detection by ELISA; routine counting of peripheral white blood cells. Results On the first day and the eighth day after admission, the serum CuZn-SOD content and the concentration of calcium and phosphorus in the crew group were significantly lower than those in the control group. The concentrations of calcium and phosphorus in urine were similar to those of the serum in the crew group. Serum CK, CK-MB and α-HB-DH activity of the crew members were significantly higher than those of the control group on the first day of admission, and the value of the eighth day was similar to that of the control group. There was no significant difference in LDH activity between the two groups on the first day (P <0.05). The content of TNF-αin the 8th day was significantly higher than that in the control group, while the content of IL-2 in the crew was significantly lower than that in the control group. On the first day, the number of white blood cells was significantly higher than that of the control group, while the eighth day was similar to the control group. Conclusion Under tropical storm conditions by the sea?