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以１８年生杉木优树嫁接苗（一年生）为外植体进行离体培养，茎段的最高分化率为６９％，侧芽的最高分化率为４０％。以茎段为外植体，在改良的ＭＳ（氮元素减半）＋ＢＡＰ２．０ｍｇ／Ｌ＋ＩＢＡ１．０ｍｇ／Ｌ的培养基上，平均每个茎段可诱导不定芽３～４个。在继代培养中，以改良的ＧＤ培养基加高浓度的ＢＡＰ６．７ｍｇ／Ｌ，平均每个茎段可诱导不定芽１１～１３个，然后在ＭＳ培养基上生长；在继代培养中激素浓度一般由低到高，再由高到低的循环，可以明显提高繁殖率。在生根培养中，以ＭＳ培养基（氮元素减半）加ＩＢＡ１．５ｍｇ／Ｌ，根的诱导率在４４％。
The 18-year-old Chinese fir good grafted seedlings (annual) as explants in vitro culture, the stem segment of the highest differentiation rate of 69%, lateral buds the highest differentiation rate of 40%. Using stem segments as explants, 3 ~ 4 adventitious buds could be induced per stem on the modified MS (N + N +) + BAP2.0mg / L + IBA1.0mg / L medium. In the subculture, the modified GD medium with high concentration of BAP6.7mg / L, the average of each stem can induce adventitious buds 11 to 13, and then grown in MS medium; in the subculture of hormones Concentrations are generally from low to high, then from high to low cycle, can significantly improve the reproduction rate. In the rooting culture, the MS medium (nitrogen element halved) plus IBA1.5mg / L, the induction rate of roots 44%.