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目的 探讨p120ctn在非小细胞肺癌组织中的表达及与各临床病理因素和预后的关系。方法 应用p120ctn单克隆抗体,采用免疫组织化学SP法检测143例非小细胞肺癌p120ctn表达情况,其中36例同时应用Westernblot方法对其蛋白表达和亚型情况进行了观察。结果 免疫组织化学:正常支气管黏膜细胞p120ctn表达为细胞膜强阳性,而在非小细胞肺癌中绝大多数表现为膜表达减弱或出现胞质内异位表达,其异常表达率为79 .7% ( 114 /143 )。异常表达与组织学类型无关(P>0 .05),但与肿瘤分化程度、TNM分期和淋巴结转移相关(P<0 .05),与非小细胞肺癌患者不良预后正相关。胞质的异位表达更容易出现在胞膜表达减弱的病例中,鳞癌中胞质异位表达高于腺癌。Westernblot:正常组织中p120ctn总蛋白量明显高于非小细胞肺癌,二者差异有统计学意义(P=0 .003)。正常肺组织p120ctn以亚型1(相对分子质量120 000)和亚型3(相对分子质量100 000)表达为主,而肿瘤组织中则以亚型1表达缺失或减弱为主。结论 p120ctn在非小细胞肺癌组织有异常表达,可作为评估预后的辅助指标。
Objective To investigate the expression of p120ctn in non-small cell lung cancer and its relationship with clinicopathological factors and prognosis. Methods The p120ctn monoclonal antibody was used to detect the expression of p120ctn in 143 cases of non-small cell lung cancer by immunohistochemical SP method. The expression of the protein and subtype of 36 cases were observed by Western blotting. Results Immunohistochemistry: The expression of p120ctn in normal bronchial mucosa cells was strongly positive in the cell membrane, while the expression of p120ctn in the normal bronchial mucosa was weakly positive or in the cytoplasm. The abnormal expression rate of p120ctn in non-small cell lung cancer was 79.7% 114/143). Abnormal expression was not related to histological type (P> 0.05), but correlated with tumor differentiation, TNM stage and lymph node metastasis (P <0.05), which was positively correlated with poor prognosis in patients with NSCLC. Ectopic expression of cytoplasm is more likely to occur in the membrane expression weakened cases, squamous cell cytoplasm ectopic expression of adenocarcinoma. Westernblot: The total amount of p120ctn protein in normal tissues was significantly higher than non-small cell lung cancer, the difference was statistically significant (P = 0.003). The expression of p120ctn in normal lung tissue was mainly expressed in subtype 1 (120 000 relative molecular weight) and subtype 3 (relative molecular mass 100 000), whereas the expression of subtype 1 was absent or diminished in tumor tissues. Conclusion p120ctn is abnormally expressed in non-small cell lung cancer tissues and can be used as a secondary indicator to evaluate prognosis.