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对甘蓝夜蛾核型多角体病毒（ ＭｂＮＰＶ） 在同源寄主细胞系ＮＥＡＵＭｂ９３１１０４（ Ｍｂ９３１１０４） 中的生长特性进行了研究。结果表明，无包涵体游离病毒（ ＭｂＮＰＶ＿ＮＯＶ） 在接种后１４ ｈ 达到吸收高峰，病毒滴度降到最低值５－７９ ×１０２ＴＣＩＤ５０／ｍ Ｌ，接种病毒９６ ｈ，病毒滴度达到最高值２－１３ ×１０５ＴＣＩＤ５０／ｍＬ，在ＮＯＶ（ｎｏｎｏｃｃｌｕｄｅｄ ｖｉｒｕｓ） 连续传递２０ 代的过程中，ＭｂＮＰＶ＿ＮＯＶ 毒力随传递代数的增加而减低，并且多角体的毒力也发生了显著的变化，前几代能使甘蓝夜蛾幼虫死亡率在９０ ％ 以上，传递到１５ 代以后产生的多角体对甘蓝夜蛾的幼虫几乎没有毒力。
The growth characteristics of M. nipponense nuclear polyhedrosis virus (MbNPV) in homologous host cell line NEAUMb931104 (Mb931104) were studied. The results showed that the inclusion body free virus (MbNPV_NOV) peaked at 14 h after inoculation, the virus titer dropped to the lowest 5-79 × 102 TCID50 / m L, and the virus titer reached the highest value of 2 h × 105 TCID50 / mL. During the continuous transmission of NOV (nonocluded virus) for 20 generations, the virulence of MbNPV_NOV decreased with the increase of transfer algebra, and the virulence of polyhedrosis also changed significantly. The death rate of larvae of cabbage budworm was over 90%. The polyhedra produced after passage 15 had almost no toxicity to the larva of the cabbage budworm.