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光动力作用所产生的单线态氧分子因其有限的寿命(1μs)和有效反应距离(<10 nm),可亚细胞特异性调控细胞的不同生理活动。如质膜定位的光动力作用可导致胆囊收缩素(cholecystokinin,CCK)1型受体的永久性激活,及其它G蛋白耦联受体的增敏或脱敏。近年来涌现出来的基因编码的蛋白质光敏剂,使得光动力作用的亚细胞定位变得更加精细可控,因而可实现亚细胞部位及功能蛋白的靶向光动力调控。蛋白质光敏剂毒杀红(KillerRed)、迷你单(miniSOG)、单蛋敏(singlet oxygen protein photosensitiser,SOPP)等的亚细胞定位表达,可光动力调控细胞局部生理过程,通过对特定蛋白的光氧化活性改变,阐明该蛋白在细胞生理过程中的作用。选择性光照技术,可实现同步多点局部光照。多点局部光动力作用调控,可阐明细胞局部对整体细胞,及个体细胞对细胞团块的影响。蛋白质光敏剂的光动力作用,正逐渐成为细胞生理学研究的重要纳米调控工具。
The singlet oxygen molecules generated by photodynamic action can regulate the different physiological activities of cells due to their limited lifespan (1μs) and effective reaction distance (<10 nm). Photodynamic effects such as plasma membrane localization can result in permanent activation of cholecystokinin (CCK) type 1 receptors and sensitization or desensitization of other G protein-coupled receptors. In recent years, gene coding protein photosensitizer has emerged, making photodynamic subcellular localization becomes more fine and controllable, which can achieve subcellular location and functional protein targeting photodynamic regulation. KillerRed, miniSOG, SOPP and other subcellular localization expression can be photodynamic control of cell local physiological processes, through the specific protein photooxidation Changes in activity, clarify the role of the protein in cell physiology. Selective lighting technology, can be achieved simultaneously multi-point local lighting. Multi-point local photodynamic regulation, can clarify the local cells on the whole cell, and individual cells on the impact of cell mass. The photodynamic action of protein photosensitizers is becoming an important tool for the regulation of cell physiology.