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采用超高效液相色谱-质谱联用法对橘子、树莓、甘蓝和菠菜中的6种有机酸(酒石酸、苹果酸、柠檬酸、琥珀酸、富马酸和马来酸)进行检测。选用ACQUITY UPLC~;HSST3色谱柱进行分离,0.1%甲酸/乙腈溶液-0.1%甲酸/水体系作为流动相进行梯度洗脱,流速为0.25 m L/min,选择ESI-多反应监测模式进行扫描。6种有机酸的平均回收率为77.9%~104.0%,相对标准偏差RSD值为2.0%~12.4%,相关系数R~2>0.998 9,该方法的最低检出限的分别为0.02 mg/kg和0.05 mg/kg,最低定量限分别为0.1 mg/L和0.25 mg/L。准确、高效地测出了四种果蔬中有机酸的含量。
Six organic acids (tartaric acid, malic acid, citric acid, succinic acid, fumaric acid and maleic acid) in orange, raspberry, cabbage and spinach were detected by ultra performance liquid chromatography-mass spectrometry. ACQUITY UPLC ~ ; HSST3 was used for separation. The mobile phase consisted of 0.1% formic acid / acetonitrile-0.1% formic acid / water with gradient elution at a flow rate of 0.25 mL / min. . The average recoveries of six organic acids ranged from 77.9% to 104.0%. The relative standard deviations (RSDs) ranged from 2.0% to 12.4% with correlation coefficients R 2> 0.998 9. The minimum detectable limits of the six organic acids were 0.02 mg / kg And 0.05 mg / kg, respectively. The lowest limit of quantification was 0.1 mg / L and 0.25 mg / L, respectively. Accurate and efficient measurement of the four kinds of fruits and vegetables in the organic acid content.