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目的:研究大孔吸附树脂分离黄粉虫黄酮的工艺条件及其抗氧化功能。方法:比较4种树脂对黄粉虫黄酮的静态吸附和解吸性能,筛选出XDA-1型大孔吸附树脂用于分离黄粉虫黄酮,并测定纯化后黄粉虫黄酮的抗氧化性。以芦丁为阳性对照。结果:最佳工艺条件是:上样液质量浓度1.0 mg/mL、吸附流速2 BV/h、上样液pH5、上样量2 BV、洗脱流速2 BV/h、乙醇体积分数60%、洗脱液体积3 BV。经分离后黄粉虫黄酮纯度由3.74%提高到16.34%,纯化倍数为4.37。黄粉虫黄酮对羟基自由基、超氧阴离子自由基均有较强的清除作用,抗脂质体过氧化优于芦丁。结论:采用XDA-1型大孔吸附树脂分离黄粉虫黄酮操作简单、安全、成本低,有较高应用价值。黄粉虫黄酮具有较高的抗氧化能力,是1种天然有效的抗氧化剂。
Objective: To study the process conditions and the anti-oxidative function of flavonoids isolated from macrofungi by macroporous adsorption resin. Methods: The static adsorption and desorption properties of flavonoids from four kinds of resins were compared. XDA-1 macroporous resin was screened for the isolation of flavonoids from Tenebrio molitor. The antioxidant activity of purified flavonoids from Tenebrio molitor was determined. Rutin as a positive control. Results: The optimum conditions were as follows: 1.0 mg / mL of sample solution, 2 BV / h of adsorption, pH5, sample volume of 2 BV, elution flow rate of 2 BV / h, ethanol volume fraction of 60% Eluent volume 3 BV. After isolation, the purity of Tenebrio flavone increased from 3.74% to 16.34%, and the purification fold was 4.37. Tenebrio flavonoids on the hydroxyl radicals, superoxide anion free radicals have a strong scavenging effect, lipid peroxidation is better than rutin. Conclusion: The separation of Tenebrio flavone with XDA-1 macroporous resin is simple, safe, low cost and has high application value. Tenebrio flavonoids with high antioxidant capacity, is a natural and effective antioxidant.