论文部分内容阅读
[目的]揭示不同耐旱性甘薯苗期根系的抗氧化特性。[方法]以耐旱性强的济薯21和耐旱性弱的济紫薯1为材料,采用水培和PEG-6000模拟干旱胁迫的方法 ,研究了干旱条件下不同耐旱性甘薯苗期根系活性氧、抗氧化物质和抗氧化酶差异。[结果]在干旱条件下,济紫薯1苗期根系的H_2O_2含量和O~(2-)生成速率分别比济薯21的高1.9%和102.6%,济薯21和济紫薯1根系中的Vc含量分别增加了25.3%和81.1%,而多酚含量则增加了24.8%和37.6%,在上述指标中济紫薯1的增幅均高于济薯21;干旱条件下,济薯21和济紫薯1根系中的抗氧化酶活性均升高,其中POD活性增幅最大,PPO活性增幅最小,济薯21苗期根系中的SOD、POD、APX和PPO活性增幅比济紫薯1的高一倍左右,两个品种干旱处理间的SOD3、POD2、CAT1、APX2和PPO2-等同工酶的条带变化明显。[结论]耐旱性越强的甘薯品种,干旱条件下苗期根系中H_2O_2含量、O~(2-)-生成速率、Vc和多酚含量增幅越小,抗氧化酶活性增幅越高,抗氧化酶差异主要与SOD3、POD2、CAT1、APX2和PPO2-等同工酶的变化有关。
[Objective] The research aimed to reveal the antioxidant activity of different drought-tolerant sweetpotato roots. [Method] With drought-resistant Jixin 21 and low-drought-tolerant Jizhu potato 1 as materials, the effects of drought stress on drought tolerance of sweet potato under seedling stage Root reactive oxygen species, antioxidants and antioxidant enzymes differences. [Result] Under drought conditions, the contents of H_2O_2 and O_ (2-) in roots of JZY1 were 1.9% and 102.6% higher than that of JZ 21 respectively, Vc content increased by 25.3% and 81.1%, respectively, while polyphenol content increased by 24.8% and 37.6% respectively. The growth of Jizhi potato 1 was higher than that of Jishu 21 in the above indexes. Under drought conditions, The activities of antioxidant enzymes in the roots of Ziyuzi were increased, and the activities of POD increased most and the activities of PPO increased least. The activities of SOD, POD, APX and PPO in roots of JZ 21 were higher than that of Ziyu 1 About one time, the bands of SOD3, POD2, CAT1, APX2 and PPO2- isozymes between the two cultivars were significantly changed. [Conclusion] The sweet potato varieties with stronger drought resistance had the lower increase of H 2 O 2 content, O 2 - production rate, Vc and polyphenol content in root system under drought condition, the higher the increase of antioxidant enzyme activity, The differences of oxidase were mainly related to the changes of isozymes such as SOD3, POD2, CAT1, APX2 and PPO2-.