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目的了解北京地区婴幼儿急性腹泻病例5种腹泻相关病毒的感染状况。方法采集北京地区哨点医院2016年1~12月<60月龄急性腹泻患儿便标本,用实时荧光PCR对A组轮状病毒、诺如病毒、肠道腺病毒、星状病毒进行检测;同时用巢式PCR检测人博卡病毒。结果共采集354份粪便标本,A组轮状病毒检出率13.84%,诺如病毒检出率10.45%,肠道腺病毒检出率4.52%,星状病毒检出率3.95%,人博卡病毒检出率7.34%。混合感染18例,占5.08%。轮状病毒发病高峰为冬季,诺如病毒的发病高峰为春季,其他病毒感染无明显季节特征。2岁以内患儿5种病毒的检出率分别为81.63%、78.38%、81.25%、71.43%、84.62%。结论北京地区急性腹泻患儿5种腹泻相关病毒检出率从高到低依次为:A组轮状病毒、诺如病毒、人博卡病毒、肠道腺病毒、星状病毒;2岁以内患儿是病毒性腹泻的高发人群。
Objective To understand the infection status of five diarrhea related viruses in infants and young children with acute diarrhea in Beijing. Methods The specimens of children with acute diarrhea who were <60 months old from January to December 2016 in sentinel hospital of Beijing were collected and detected by real-time fluorescence PCR for group A rotavirus, norovirus, enterovirus and astrovirus. At the same time using nested PCR detection of human Boka virus. Results A total of 354 stool specimens were collected. The detection rate of rotavirus in group A was 13.84%, that of Norovirus was 10.45%, that of intestinal adenovirus was 4.52%, that of astrovirus was 3.95% Virus detection rate of 7.34%. Mixed infection in 18 cases, accounting for 5.08%. Rotavirus peak incidence in winter, norovirus onset peak in spring, no other seasonal virus infection characteristics. The detection rates of 5 viruses within 2 years of age were 81.63%, 78.38%, 81.25%, 71.43% and 84.62% respectively. Conclusions The detection rates of the five diarrhea-related viruses in children with acute diarrhea in Beijing are as follows: group A rotavirus, norovirus, human boca virus, enteric adenovirus and astrovirus; Children are high risk of viral diarrhea.