【摘 要】
:
The development of cell replacement techniques is promising as a potential treatment for photoreceptor loss. However, the limited integration ability of donor a
【机 构】
:
Department of Ophthalmology, Chinese PLA Genera/Hospital, Beijing 100853, China“,”Schepens Eye Resea
论文部分内容阅读
The development of cell replacement techniques is promising as a potential treatment for photoreceptor loss. However, the limited integration ability of donor and recipient cells presents a challenge following transplantation. In the present study, retinal progenitor cells (RPCs) were harvested from the neural retinas of enhanced green fluorescent protein mice on postnatal day 1, and expanded in a neurobasal medium supplemented with fetal bovine serum without endothelial growth factor. Using a confocal microscope, immunohistochemistry demonstrated that expanded RPCs in vitro maintain retinal stem cell properties and can be differentiated into photoreceptor cells. Three weeks after transplantation, subretinal transplanted RPCs were found to have migrated and integrated into the outer nuclear layer of recipient retinas with laser injury, some of the integrated cells had differentiated into photoreceptors, and a subpopulation of these cells expressed photoreceptor specific synaptic protein, appearing to form synaptic connections with bipolar cells. These results suggest that subretinal transplantation of RPCs may provide a feasible therapeutic strategy for the loss of retinal photoreceptor cells.
其他文献
目的 观察硫酸镁联合硝苯地平治疗妊娠高血压的治疗效果.方法 63例妊娠高血压患者,根据药物治疗方法不同分为观察组(32例)和对照组(31例).对照组给予硫酸镁治疗,观察组给予在
Proton magnetic resonance spectroscopy (1H-MRS) permits the assessment of cerebral neurometabolites, such as N-acetylaspartate, choline, and creatine, in vivo a
Brachial plexus birth injuries in children are usually diagnosed using 1.5-T magnetic resonance imaging, while the application of high-field magnetic resonance
目的:分析育龄女性孕前血压水平与低出生体质量发生风险的关系。方法采用巢式病例对照研究的方法,将2013年参加国家免费孕前优生健康检查的育龄妇女纳入孕前队列,以随访期内生育
目的:分析广东省不同区域、不同级别医疗机构2005—2013年剖宫产率趋势并预测2014、2015年剖宫产率,为降低剖宫产率相关措施的制定提供参考依据。方法采用 Mann-Kendall 法对广
目的:探讨轻-中度持续性哮喘患儿应用沙美特罗替卡松治疗的依从情况,分析其影响因素。方法36例轻-中度持续性哮喘患儿给予低剂量沙美特罗替卡松粉吸入,每吸100μg,早、晚各1吸,共1
Previous research has demonstrated that cotransplantation of umbilical cord mesenchymal stem cells (UCMSCs) and Schwann cells (SCs) can repair spinal nerve inju
Exogenous stem cell transplantation and endogenous stem cell mobilization are both effective for the treatment of acute cerebral infarction. The compound dl-3-b
目的:分析加温二氧化碳气腹对妇科腹腔镜手术患者血气的影响及手术麻醉干预措施。方法选取2012年5月到2014年5月于深圳市妇幼保健院妇科接受腹腔镜手术治疗的120例患者作为研
Some evidence suggests that multiwalled carbon nanotubes can promote neuronal differentiation and growth. The present study investigated the effect of functiona