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探讨转化人胚腱细胞 (Transformedhumanembryonictendoncells,THETCs)力学特性 ,以阐明在应变条件下进行细胞 -支架材料复合培养时腱细胞的力学相容性。取人胚腱细胞 (Humanembryonictendoncells,HETCs)和THETCs ,采用微吸管吸吮实验技术 ,测量单个细胞的粘弹性及其对聚乳酸 (Polylacticacid,PLA)和聚乳酸羟基乙酸共聚物85/15(Polylactic -co - glycolicacid,PLGA85/15)的粘附力。在小变形条件下 ,HETC剪切弹性模量 (均值87.4Pa)和粘性系数(均值7.4Pa.S)分别小于THETC的剪切弹性模量 (均值115.2Pa)和粘性系数 (均值12.7Pa.S)。HETC在PLA和PLGA85/15膜表面的切向粘附力分别为2.38×10-8N和1.61×10-8N ,THETC在PLA和PLGA85/15膜表面的切向粘附力分别为0.98×10 -8N和1.57×10 -8N。与HETC相比 ,THETC具有较强的抗剪切能力 ,与PLGA85/15膜表面粘附性较好。提示 ,选择THETC与PLGA85/15支架材料复合 ,细胞在材料表面力学相容性好 ,满足应变条件下细胞 -支架材料复合培养对细胞的力学要求。
To investigate the mechanical properties of transformed human embryonic tenocytes (THETCs) in order to elucidate the mechanical compatibility of tendon cells under the condition of cell-scaffold material culture under strain conditions. Humanembryonic tendon cells (HETCs) and THETCs were harvested and the viscoelasticity of single cells and their effect on polylactic acid (PLA) and polylactic acid glycolic acid copolymer 85/15 (Polylactic-co - glycolic acid, PLGA 85/15). Under small deformation conditions, the shear modulus of elasticity (mean value 87.4Pa) and viscosity coefficient (mean value 7.4Pa.S) of HETC are less than those of THETC (average 115.2Pa) and viscosity coefficient (mean 12.7Pa.S) ). The tangential adhesion of HETC on PLA and PLGA85 / 15 membranes was 2.38 × 10-8 N and 1.61 × 10-8 N, respectively. The tangential adhesion of THETC to PLA and PLGA85 / 15 membranes was 0.98 × 10 - 8N and 1.57 × 10 -8N. Compared with HETC, THETC possesses strong anti-shear ability and good adhesion with PLGA85 / 15 membrane surface. Tip, choose THETC and PLGA85 / 15 scaffold material composite, the mechanical compatibility of cells in the material surface to meet the cell strain of cell-scaffold material under the mechanical requirements of cells.