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目的比较不同新生牛血清对体外培养的BHK-21细胞的影响。方法以不同厂家和同一厂家不同批次的共6种新生牛血清培养BHK-21细胞,观察细胞传代后在不同血清培养条件下的形态、增长倍数和细胞维持时间。结果 6号血清培养的细胞生长状态最好,增长倍数最高,维持细胞存活时间最长;3号血清培养效果次之;2号、4号和5号血清培养的细胞至第4天仍未长满单层;1号血清培养的细胞基本未生长。结论不同厂家和同一厂家不同批次的新生牛血清对BHK-21细胞体外培养的效果存在差异。
Objective To compare the effects of different neonatal bovine serum on BHK-21 cells cultured in vitro. Methods BHK-21 cells were cultured in 6 newborn calluses of different batches from different manufacturers and different batches of the same manufacturer. Morphology, growth multiples and cell maintenance time were observed under different serum culture conditions after passage. Results The number of cells cultured in serum 6 was the best, with the highest growth rate and the longest survival time. The effect of culture on number 3 was second, and the number of cells cultured in serum of No. 2, 4 and 5 was not longer Monolayer; cells cultured in serum # 1 did not grow. Conclusion Different batches of newborn calf bovine serum from different manufacturers and same manufacturers have different effects on the in vitro culture of BHK-21 cells.