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目的：探讨三叶因子3（TFF3）在腺垂体远侧部嗜酸性细胞和嗜碱性细胞中的表达，明确TFF3在远侧部的分布。方法采用相邻切片的免疫组织化学染色，在相邻切片上分别显示TFF3/生长激素（ GH）、TFF3/催乳素（PRL）、TFF3/促甲状腺激素（TSH）、TFF3/促肾上腺皮质激素（ACTH）、TFF3/卵泡刺激素（FSH）、TFF3/黄体生成素（ LH）的表达。结果 TFF3和各嗜色细胞的免疫反应产物为棕黄色，主要位于细胞质，ACTH免疫阳性信号在胞膜也有表达，主要分布在腺垂体的远侧部。邻片显示TFF3存在于部分GH、PRL、TSH、ACTH、FSH、LH细胞，分别占19．4％、22．4％、9．2％、6．5％、35．7％、8．3％，以FSH最多，PRL、GH次之。结论垂体远侧部TFF3可分别表达于GH、PRL、TSH、ACTH、FSH、LH细胞。“,”Objective To investigate the distribution and expression of trefoil factor 3(TFF3) in the eosinophilic cells and basophilic cells in the pars distalis of the rat pituitary .Methods The immunohistochemiscal staining technique was used to show the coexpression of TFF3/growth hormone (GH),TFF3/prolactin (PRL),TFF3/thyrotroph (TSH), TFF3/adrenocorticotropin (ACTH),TFF3/follicle stimulating hormone (FSH),TFF3/luteinizing hormone (LH) in two contiguous slices .Results The immunoreactive products of TFF 3 and chromophil cells were brown granules , mainly expressed in cytoplasm .ACTH positive signals were expressed in the cell membrane and mainly located in the pars distalis hypophyseos.TFF3 existed in parts of GH,PRL,TSH,ACTH,FSH and LH cells in contiguous slices, accounting for 19.4%, 22.4%, 9.2%, 6.5%, 35.7%, 8.3%, respectively , in which FSH was the most , PRL and GH were less . Conclusion TFF3 expresses in GH, PRL, TSH, ACTH, FSH, and LH cells in the pars distalis , which enriches the morphological data for the location of TFF 3 in gland cells of pars distalis hypophyseos .