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目的探讨姜黄素(Curcumin)对脓毒血症诱导的心肌功能障碍(Sepsis-induced myocardial dysfunction,SIMD)小鼠转化生长因子-β1(Transforming growth factor beta-1,TGF-β1)表达的影响。方法将SD小鼠随机分为假手术组(Sham组)、脓毒血症组(Sep组)、二甲基亚砜组(DMSO组)和姜黄素组(Cur组),Sep、DMSO和Cur组采用盲肠结扎穿刺术(Cecal ligation andpuncture,CLP)复制SIMD模型,造模24 h后,Cur组经腹腔注射200 mg/(kg.d)姜黄素,Sham和Sep组经腹腔注射等量生理盐水,DMSO组经腹腔注射等量DMSO。造模成功后0、6和12 h,各组小鼠经从右颈动脉插管使用BL-410多功能监护仪监测左心室收缩压(Left ventricular systolic pressure,LVSP)、左心室舒张末期压(Left ventricular end-diastolic pressure,LVEDP)、左室内压力变化最大上升和下降速率(Maximum rate of left ventricular pressure development and decay,LV±dp/dt-max);HE染色观察心肌组织病理变化;Western blot检测心肌组织中TGF-β1蛋白的表达。结果 Cur组小鼠24和72 h存活率均高于Sep组;心脏血流动力学指标(LVSP、LVEDP和LV±dp/dt-max)在各时间点与Sep组比较,均明显上升(P<0.05或P<0.01);各种心肌损害的病理学改变与Sep组相比,从6 h开始明显减轻;心肌组织中TGF-β1蛋白的表达量较Sep组明显下降(P<0.05)。各指标DMSO组与Sep组比较,差异均无统计学意义(P>0.05)。心肌病理学改变、心脏血流动力学变化及TGF-β1蛋白表达变化在各时相点存在一致性。结论姜黄素对SIMD小鼠的心肌保护作用与抑制TGF-β1蛋白的表达有关。
Objective To investigate the effect of Curcumin on the expression of Transforming growth factor beta-1 (TGF-β1) in sepsis-induced myocardial dysfunction (SIMD) mice. Methods SD mice were randomly divided into Sham group, Sep group, dimethyl sulfoxide group and Cur group, Sep, DMSO and Cur SIMD model was replicated by Cecal ligation and CLP (CLP). After 24 h of modeling, curcumin (200 mg / (kg.d)) was injected intraperitoneally in group Cur and intraperitoneal injection of equal volume of normal saline DMSO group was injected intraperitoneally with the same amount of DMSO. At 0, 6, and 12 h after successful modeling, the mice in each group were anesthetized by BL-410 multi-function monitor from the right carotid artery. Left ventricular systolic pressure (LVSP), left ventricular end-diastolic pressure Left ventricular end-diastolic pressure (LVEDP) and left ventricular pressure (LVEDP) and left ventricular pressure (LVEDP) were measured. The pathological changes of myocardium were observed by HE staining. Expression of TGF-β1 in Myocardial Tissue. Results The survival rates of mice in Cur group at 24 h and 72 h were higher than those in Sep group. The indexes of hemodynamics (LVSP, LVEDP and LV ± dp / dt-max) in Cur group were significantly higher than those in Sep group at each time point <0.05 or P <0.01). The pathological changes of various myocardial lesions were significantly reduced from 6 h after the onset of myocardial injury. The expression of TGF-β1 protein in myocardium was significantly lower than that in Sep group (P <0.05). There was no significant difference between DMSO group and Sep group (P> 0.05). Changes in myocardial pathology, changes in cardiac hemodynamics and TGF-β1 protein expression at various time points there is consistency. Conclusion The protective effect of curcumin on SIMD mice is related to the inhibition of TGF-β1 protein expression.