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目的探讨降低大脑中动脉阻塞(middle cerebral artery occlusion,MCAO)模型大鼠病死率的有效方法。方法165只大鼠随机分成插线18mm组56只、插线17.3mm组54只和插线17.3mm+引流术组55只。3组均采用改良Longa法制作MCAO模型,分别于≤1d、>1~3d、>3~7d时统计大鼠死亡数,比较3组大鼠MCAO模型成功率和各时间点病死率。结果插线18 mm组、插线17.3 mm组和插线17.3 mm+引流术组MCAO模型成功率分别为89.29%、92.59%、90.91%,两两比较差异无统计学意义(P>0.05);≤1d时,3组大鼠病死率比较差异无统计学意义(P>0.05);>1~3d时、>3~7d时插线18mm组(24.0%、10.0%)和插线17.3mm组(24.0%、6.0%)大鼠病死率高于插线17.3mm+引流术组(10.0%、0)(P<0.05),插线18 mm组与插线17.3 mm组比较差异无统计学意义(P>0.05)。结论侧脑室引流术联合17.3mm插线深度能明显降低MCAO模型大鼠病死率。
Objective To explore an effective method to reduce the mortality of rats with middle cerebral artery occlusion (MCAO). Methods Sixty-five rats were randomly divided into 18 groups (56mm), 54 (17.3mm), and 17.3mm (+) drainage group. The MCAO model was made by modified Longa method in 3 and 3 groups, respectively. The number of deaths of rats was calculated at ≤1d,> 1-3d and> 3-7d, respectively. The success rate of MCAO model and mortality at each time point were compared between the three groups. Results The successful rates of MCAO model of 18 mm wire insertion group, 17.3 mm wire insertion group and 17.3 mm wire insertion + drainage group were 89.29%, 92.59% and 90.91%, respectively. There was no significant difference between any two groups (P> 0.05); There was no significant difference in the case fatality rate between the three groups at 1d (P> 0.05). In the group of> 1-3d, the 18mm group (24.0%, 10.0%) and the 17.3mm group 24.0% and 6.0%, respectively) were significantly higher than those in 17.3 mm and drainage group (10.0%, 0) (P <0.05). There was no significant difference in the fatality rate between 18 mm and 17.3 mm (P > 0.05). Conclusion Lateral ventricular drainage combined with 17.3 mm insertion depth can significantly reduce the mortality of MCAO rats.