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为了解人乳头瘤病毒（ＨＰＶ）对免疫Ｌａｎｇｅｒｈａｎｓ细胞（ＬＣ）的影响及ＨＰＶ的致癌机理，采用地高辛配基标记的ＨＰＶ１６／１８ＤＮＡ探针和抗Ｓ１００蛋白抗体，分别用原位分子杂交和免疫组化ＬＳＡＢ法，检测了４０例食管鳞状细胞癌中ＨＰＶＤＮＡ序列及分布，重点观察了ＨＰＶ感染时局部癌旁上皮内Ｓ１００蛋白阳性Ｌａｎｇｅｒｈａｎｓ细胞的分布、数量及形态改变。结果显示：在有ＨＰＶ感染时，上皮内ＬＣ数量明显少于无感染者。上皮内残留的少数ＬＣ的分布及形态也有改变。结果提示：ＨＰＶ可能通过减少局部的ＬＣ，破坏机体局部的免疫监视系统，并与其他致癌因素协同作用，进而导致食管癌的发生。
To understand the effect of human papillomavirus (HPV) on immune Langerhans cells (LC) and the carcinogenic mechanism of HPV, digoxigenin-labeled HPV16/18 DNA probe and anti-S-100 protein antibody were used, respectively. Hybridization and immunohistochemistry LSAB method was used to detect the sequence and distribution of HPV DNA in 40 cases of esophageal squamous cell carcinoma. The distribution, quantity and morphological changes of S-100 protein positive Langerhans cells in the para-cancerous epithelium of HPV infection were mainly observed. The results showed that in the presence of HPV infection, the number of intraepithelial LCs was significantly less than those without infection. The distribution and morphology of a few LCs remaining in the epithelium also changed. The results suggest that HPV may destroy the local immune surveillance system by reducing local LC, and synergistically with other carcinogenic factors, leading to the occurrence of esophageal cancer.