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制备了碳纳米管修饰玻碳电极(CNT/GC),利用吸附的方法将葡萄糖氧化酶(GOx)固定到CNT/GC电极表面,形成GOx-CNT/GC电极,研究了GOx的直接电子转移,实验结果表明,GOx在CNT/GC电极表面没有发生变性,能进行有效和稳定的直接电子转移反应,其循环伏安图上表现出一对很好的、几乎对称的氧化还原峰;式量电位E~0几乎不随扫速(至少在10～140 mV·s~(-1)的扫速范围内)而变化,其平均值为-0.456±0.0008 V(vs.SCE);GOx在CNT/GC电极表面直接电子转移的速率常数为1.74±0.42 s~(-1),比文献中报道的值大了数十倍;进一步的实验结果显示,固定在CNT/GC电极表面的GOx能保持其对葡萄糖氧化的生物电催化活性,而且电催化活性很稳定。文中制备碳纳米管修饰电极和固定酶的方法具有简单和易于操作等优点,可用于获得其他生物氧化还原蛋白质和酶的直接电子转移。
The carbon nanotubes modified glassy carbon electrode (CNT / GC) was prepared. The glucose oxidase (GOx) was immobilized on the surface of CNT / GC electrode by adsorption method to form GOx-CNT / GC electrode. The direct electron transfer The experimental results show that GOx does not degenerate on the surface of CNT / GC electrode, and can perform effective and stable direct electron transfer reaction. The cyclic voltammogram of GOx shows a pair of very good and nearly symmetric redox peaks. E 0 hardly changed with the sweep rate (at least within the range of 10 ~ 140 mV · s -1), and the average value was -0.456 ± 0.0008 V (vs.SCE) The rate constant of direct electron transfer on the electrode surface was 1.74 ± 0.42 s ~ (-1), which was several times larger than the reported value in the literature. Further experimental results showed that the GOx immobilized on the surface of CNT / Bioelectrocatalytic activity of glucose oxidation, and electrocatalytic activity is very stable. The method of preparing carbon nanotubes modified electrodes and immobilized enzymes in the paper has the advantages of simple and easy operation and the like, and can be used for obtaining the direct electron transfer of other biological redox proteins and enzymes.