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目的:了解2009年-2010年浙江省义乌市手足口病(HFMD)的病原体型别及分布特征,为今后制定预防和控制HFMD防治策略提供依据。方法:采用实时荧光RT-PCR方法,对义乌市248例HFMD临床诊断病例标本进行EV、EV71和CoxA16RNA的检测。结果:共检出178例EV阳性病例,阳性率为71.77%;其中EV71阳性病例83例,CoxA16阳性病例66例,分别占EV阳性病例的46.63%、37.08%;5岁以下儿童EV感染占所有感染者的92.74%;2009年和2010年儿童EV71、CoxA16阳性率分别为17.86%、37.50%和38.05%、23.44%,结果差别有统计学意义;不同性别儿童EV、EV71和CoxA16阳性率差别无统计学意义;重症病例EV71阳性检出率为75.00%。结论:引起义乌市HFMD的主要病原体为EV71和CoxA16,其中2009年多数为CoxA16,其次为EV71,2010年多数为EV71、其次为CoxA16;5岁以下儿童是EV感染的高危人群;EV71是引起重症病例的主要病原体。
Objective: To understand the types and distribution of pathogens of HFMD in Yiwu City, Zhejiang Province from 2009 to 2010, and to provide basis for future prevention and control strategies of HFMD. Methods: The real-time fluorescent RT-PCR method was used to detect EV, EV71 and CoxA16RNA in 248 cases of HFMD diagnosed in Yiwu City. Results: A total of 178 EV positive cases were detected, with a positive rate of 71.77%. Among them, 83 cases were EV71 positive and 66 cases were CoxA16 positive, which accounted for 46.63% and 37.08% respectively of EV positive cases. EV infection accounted for The positive rates of EV71 and CoxA16 in children in 2009 and 2010 were 17.86%, 37.50% and 38.05%, 23.44%, respectively. The difference was statistically significant. The positive rates of EV, EV71 and CoxA16 in children of different sex were no difference Statistical significance; Severe cases EV71 positive detection rate of 75.00%. CONCLUSION: The main pathogens that cause HFMD in Yiwu are EV71 and CoxA16, most of which were CoxA16 in 2009, followed by EV71, EV71 in 2010 and CoxA16 followed by children under 5 years old at high risk of EV infection. EV71 is a major cause of severe disease The main pathogen of the case.