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[Objective]To establish methods to determine the content of Aconitine,Yunaconitine,Crassicauline and Vilmorrianine in Aconitun sungpanense Hand-Mazz.and provide scientific basis for its reasonable and safe application.[Methods]Aconitine,Yunaconitine,Crassicauline and Vilmorrianine were used as references.Phenomenex Gemini C18110A(250 mm×4.6 mm,5μm);mobile phase:acetonitrile-0.2%acetic acid(pH was regulated to by triethylamine);gradient elute;flow rate:1 mL/min;UV wavelength 235/254 nm;column temperature:30℃.[Results]The content of the 4 Ester-series alkaloids in Aconitun sungpanense Hand.-Mazz.could be determined at the same time.The average recovery rate(n=6)of Aconitine,Yunaconitine,Crassicauline and Vilmorrianine reached 99.65%(RSD=1.46%),99.13%(RSD=1.32%),98.13%(RSD=0.98%)and 98.66%(RSD=1.67%).[Conclusions]The method was simple,accurate and had good repeatability.It could determine the content of Aconitine,Yunaconitine,Crassicauline and Vilmorrianine in Aconitun sungpanense Hand.-Mazz.at the same time.It also provided scientific basis for standardizing the processing of Aconitun sungpanense Hand.-Mazz.and its reasonable and safe application.
[Objective] To establish methods to determine the content of Aconitine, Yunaconitine, Crassicauline and Vilmorrianine in Aconitun sungpanense Hand-Mazz. And provide scientific basis for its reasonable and safe application. [Methods] Aconitine, Yunaconitine, Crassicauline and Vilmorrianine were used as references .Phenomenex Gemini C18110A (250 mm × 4.6 mm, 5 μm); mobile phase: acetonitrile-0.2% acetic acid (pH was regulated to by triethylamine); gradient elute; flow rate: 1 mL / min; UV wavelength 235/254 nm; column temperature: 30 ° C. [Results] The content of the 4 Ester-series alkaloids in Aconitun sungpanense Hand-Mazz.could determined at the same time.The average recovery rate (n = 6) of Aconitine, Yunaconitine, Crassicauline and Vilmorrianine reached 99.65% (RSD = 1.46%), 99.13% (RSD = 1.32%), 98.13% (RSD = 0.98%) and 98.66% (RSD = 1.67%). [Conclusions] The method was simple, accurate and had good repeatability. It could determine the content of Aconitine, Yunaconitine, Crassicauline and Vilmorrianine in Aconitun sungpanense Han d.-Mazz.at the same time. It also provides scientific basis for standardizing the processing of Aconitun sungpanense Hand.-Mazz.and its reasonable and safe application.