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平菇是一种栽培很普遍的食用菌,其细胞结构的观察在国内外报道不多,尤其是平菇的有丝分裂和减数时染色体观察尚未见报道。笔者在这方面进行了初步观察研究,现简报如下:(一)材料方法:供试菌种为紫孢侧耳(P-leurotus sapidus),选择处于不同发育程度的子实体,从菇体刚出现菌褶开始至菌盖成熟,菌盖直径每增加0.5cm 取样一次,共取10个材料。分别用Carnoy’s 固定液固定,其时间为20～24小时。用卡宝品红染液染色,压片涂抹法制片观察,显微照像。
Pleurotus ostreatus is a kind of edible fungus which is widely cultivated. The observation of its cell structure is not reported at home and abroad, especially the chromosomal observation of mitosis and meiosis of Pleurotus ostreatus has not been reported yet. The author conducted preliminary observations in this regard, are briefings are as follows: (a) Material Methods: The test species is P-leurotus sapidus, select the fruiting bodies of different developmental levels, Pleated cover began to mature, cap diameter 0.5cm for each additional sample, a total of 10 materials. Carnoy’s fixative solution was used, the time was 20 to 24 hours. Stained with card treasure magenta dye, tablet smear smear observation, microscopy.