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目的:探讨双歧杆菌脂磷壁酸(BLTA)对B16黑色素瘤荷瘤小鼠免疫功能的影响。方法将黑色素瘤B16细胞接种于C57BL/6小鼠皮下,待触及肿块后于荷瘤小鼠皮下注射BLTA。应用免疫细胞化学法检测荷瘤小鼠外周血T淋巴细胞亚群的变化情况,MTT法检测荷瘤小鼠NK细胞杀伤活性和CTL活性,计算抑瘤率、脏器指数。结果:与对照组相比,经BLTA100和BLTA150处理后的荷瘤小鼠CD4+T细胞百分率以及CD4+/CD8+的比值均显著升高(P<0.05),NK细胞杀伤活性增强、CTL活性增加(P<0.05)。肿瘤重量均显著低于对照组(P<0.05),胸腺指数和脾脏指数明显增加(P<0.05),结论:BLTA对B16黑色素瘤荷瘤小鼠具有免疫增强作用。
Objective: To investigate the effect of Bifidobacterium lipoteichoic acid (BLTA) on immune function in mice bearing B16 melanoma. Methods Melanoma B16 cells were inoculated subcutaneously in C57BL / 6 mice and injected into tumor-bearing mice subcutaneously after BLTA. The changes of T lymphocyte subsets in peripheral blood of tumor-bearing mice were detected by immunocytochemical method. The cytotoxic activity and CTL activity of NK cells in tumor-bearing mice were detected by MTT assay. The tumor inhibition rate and organ index were calculated. Results: Compared with the control group, the percentage of CD4 + T cells and the ratio of CD4 + / CD8 + of BL21 and BLTA150 treated mice were significantly increased (P <0.05), and the cytotoxicity of NK cells increased and the activity of CTL increased P <0.05). The tumor weight was significantly lower than that of the control group (P <0.05), and the thymus index and spleen index were significantly increased (P <0.05). Conclusion: BLTA has an immune enhancing effect on B16 melanoma tumor-bearing mice.