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用已知方法从牛角椒(Capsicum annuum)新鲜果实中提取含油树脂,上硅胶柱,用正己烷-乙酸乙酯梯度洗脱。正己烷-乙酸乙酯(80:20)部分上反相硅胶柱,75％甲醇洗脱,得到含有化合物1的混合物。混合物经制备型HPLC纯化:采用ODS-H80柱,80％甲醇内加0.05M硝酸银为洗脱剂,流速8.0ml/min,荧光检测。具有特征峰的部分用氯仿萃取3次,无水硫酸
The oleoresin was extracted from the fresh fruit of Capsicum annuum by known methods and applied to a silica gel column, which was eluted with a n-hexane-ethyl acetate gradient. A n-hexane-ethyl acetate (80:20) portion was eluted on a reverse phase silica gel column with 75% methanol to give a mixture containing Compound 1. The mixture was purified by preparative HPLC using an ODS-H80 column, 80% methanol plus 0.05 M silver nitrate as eluent, flow rate 8.0 ml/min, fluorescence detection. The fraction with characteristic peaks was extracted with chloroform three times, anhydrous sulfuric acid