目的使用具有甲苯降解能力的假单孢菌株作为生物催化剂,通过不对称氧化反应实现手性4-氯二苯甲醇的对应选择性合成。方法采用悬浮细胞生物转化法用于4-氯二苯甲烷的不对称氧化反应研究,产物通过手性液相色谱进行分析。结果得到了5.5%的产率和具有89%的对映选择性的手性4-氯二苯基甲醇。结论通过生物催化实现了4-氯二苯甲烷苄位C-H键的不对称羟基化反应,合成了手性的4-氯二苯基甲醇。 Objective To synthesize chiral 4-chlorobenzyl alcohol by asymmetric oxidation using Pseudomonas sp. Strain with toluene degradation as a biocatalyst. Methods Asymmetric oxidation of 4-chlorodiphenylmethane with suspension cell biotransformation method was studied. The product was analyzed by chiral liquid chromatography. The result was a 5.5% yield and an enantioselective chiral 4-chlorodiphenylmethanol with 89%. Conclusion The asymmetric hydroxylation of 4-chlorobenzylbenzyl C-H bond was achieved by biocatalysis, and chiral 4-chlorodiphenylmethanol was synthesized.