Breast cancer has been the most common and lethal cancer among women worldwide for many years, with about 1.6 million new cases worldwide each year, and 500,000 deaths from breast cancer. A large number of studies have shown that biomarkers can be used for the diagnosis of various diseases, miRNA is related to the abnormal expression, occurrence, development and efficacy of breast cancer, suggesting that it can be used as a biomarker for screening and diagnosis of diseases. Graphene-based biosensor, due to its advantages, such as high sensitivity and specificity, rapid analysis, low cost, good biological compatibility, miniaturization and integration and so on, is very suitable for the detection of tumor-related miRNA and can play an important role in the early diagnosis of breast cancer. This paper reviews the application of grapheme-based biosensor for the detection of miRNA related to breast cancer.