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研究了在 Ca2 + 存在下 ,丹磺酰标记的花粉钙调素 ( D-p Ca M)与合成的多肽相互作用的规律 .研究结果表明 ,被研究的绝大多数多肽能与 D-p Ca M结合而形成复合物 ,使 D-p Ca M的荧光光谱发生变化 .用荧光法测定了这些复合物的解离常数 Kd,其中肽 BP-1 3的 Kd 值为 4 .0 nmol/ L,是与 p Ca M结合能力最强的一个多肽 .除证明了多肽的疏水性 ,预测二级结构形成倾向和链长对其与 p Ca M结合能力有影响外 ,还发现用D-丙氨酸取代肽链中的 Gly或 L-丙氨酸后 ,可改变肽对 p Ca M的亲和性 ,这为提高多肽与钙调素的亲和力提供了一条改进的途径 .我们的研究还可以定性地说明不同来源 Ca M性质相似又有差异 ,反映了它们的高度保守性和变异性 .同时还展示了所研究的多肽对细胞信息传导过程的影响 ,为研究 Ca M对细胞功能调节的作用机制和建立 p Ca M拮抗肽的活性筛选模型提供了思路
The interaction between dansyl-labeled pollen calmodulin (Dp Ca M) and synthetic peptides was studied in the presence of Ca2 + .The results showed that most of the polypeptides studied could combine with Dp Ca M to form Complex to change the fluorescence spectrum of Dp Ca M. The dissociation constants Kd of these complexes were measured by fluorescence method and the Kd value of peptide BP-1 3 was 4.0 nmol / L, which was in combination with p Ca M One of the most potent peptides.In addition to demonstrating the hydrophobic nature of the polypeptide, predicting the secondary structure formation propensity and chain length of its impact on p Ca M binding capacity, but also found that the D-alanine substitution of peptide Gly Or L-alanine can change the affinity of the peptide for pCaM, which provides an improved way to improve the affinity of polypeptide with calmodulin.Our study can also qualitatively explain the CaM properties of different sources Similarities and differences, reflecting their high degree of conservation and variability. At the same time also demonstrated the study of the polypeptide on cell signaling process, in order to study the role of Ca M on cell function regulation mechanism and the establishment of p Ca M antagonistic peptide Provides an idea of the active screening model