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目的研究丙型肝炎病毒(HCV)核心蛋白对人源性肝细胞凋亡的影响。方法构建HCV核心蛋白真核表达载体pIRES-core,转染人源永生化肝细胞QSG7701,Western blotting印记法检测核心蛋白及转录因子NF-κB的表达;流式细胞仪检测细胞凋亡率。结果 QSG7701/core组细胞凋亡率为(1.34±0.07)%,低于QSG7701组(2.35±0.11)%和QSG7701/pcDNA3.1组(2.58±0.13)%,三组比较差异有统计学意义(P<0.05);同时转录因子NF-κB表达上调。结论 HCV核心蛋白上调转录因子NF-κB的表达,可能是抑制人源性肝细胞QSG7701凋亡的主要机制。
Objective To study the effect of hepatitis C virus (HCV) core protein on human hepatocyte apoptosis. Methods The eukaryotic expression vector pIRES-core of HCV core protein was constructed and transfected into human immortalized hepatocytes QSG7701. The expression of core protein and transcription factor NF-κB was detected by Western blotting, and the apoptosis rate was detected by flow cytometry. Results The apoptosis rate of QSG7701 / core group was (1.34 ± 0.07)%, lower than that of QSG7701 group (2.35 ± 0.11)% and QSG7701 / pcDNA3.1 group (2.58 ± 0.13)%, the difference was statistically significant P <0.05). At the same time, the transcription factor NF-κB was up-regulated. Conclusion HCV core protein up-regulates the expression of NF-κB, which may be the main mechanism of inhibiting the apoptosis of human hepatocyte QSG7701.