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1960年,Rosenberg等首先使用水溶性塑料包埋剂一乙二醇甲基丙烯酸酯(Glycol methac-rylate,缩写为GMA)作为电镜包埋剂以后,GMA的使用价值逐步受到重视,并从电镜的应用,推广到光学显微镜、组织化学等各个领域。GMA包埋的组织块,很少收缩,切成1-3μ的半薄切片后,在光镜下,几乎无细胞重叠现象,物质的分布与酶的定位清晰可见,克服了石蜡切片的收缩假象与新鲜冰冻切片质量不易保证等缺点。Feder等在应用塑料包埋技术于组织化学方面,做了许多工作。尤其是Higuchi(1979)和Namba(1983)等,在GMA包埋的切片上,进行了较详细酶活性的观察。近年来,国内也开展了GMA包埋的切片技术,张承志等做了粘多糖的
In 1960, Rosenberg and other first use of water-soluble plastic embedding agent glycol (Glycol methac-rylate, abbreviated as GMA) as electron microscopy embedding agent, the value of the use of GMA gradually attention and electron microscopy Applications, to promote the optical microscope, tissue chemistry and other fields. GMA-embedded tissue blocks, rarely shrink, cut into 1-3μ semi-thin sections, under light microscopy, almost no cell overlap phenomenon, the distribution of the substance and the enzyme localization clearly visible, to overcome the paraffin sections of the contraction artifacts And frozen sections of fresh quality assurance is not easy and other shortcomings. Feder et al. Do a lot of work on the application of plastic embedding technique in histochemistry. In particular, Higuchi (1979) and Namba (1983) observed the more detailed enzymatic activity on GMA-embedded sections. In recent years, China also carried out the GMA embedded sectioning technology, Zhang Chengzhi made mucopolysaccharide