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目的:比较琼脂稀释法和纸片扩散法对鲍曼不动杆菌的药敏试验结果。方法:随机挑选的300株鲍曼不动杆菌,检测其标本及科室的分布情况,并采用琼脂稀释法和纸片扩散法检测鲍曼不动杆菌对环丙沙星(CIP)、庆大霉素(CN)、阿米卡星(AK)、头孢他啶(CAZ)、头孢吡肟(FEP)、左氧氟沙星(LEV)、氨苄西林-舒巴坦(SAM)、妥布霉素(TOB)、美洛培南(MEN)、米诺环素(MH)、头孢哌酮-舒巴坦(SCF)11种抗菌药物的敏感性,比较两种检测结果的差异。结果:300株鲍曼不动杆菌主要分布在痰液标本中,共214株,占71.3%,主要来源于ICU 101株(33.7%)及脑外科59株(19.7%)。药敏检测结果显示,两种检测方法所得的SCF和MH的敏感性差异具有统计学意义(P<0.05),其他9种抗菌药物的药敏检测结果差异没有统计学意义(P>0.05)。结论:琼脂稀释法和纸片扩散法对鲍曼不动杆菌药敏试验结果并不完全一致,临床用药时尤其要注意SCF和MH这两种药物药敏结果的可靠性。
OBJECTIVE: To compare the susceptibility test results of Acinetobacter baumannii by agar dilution method and disk diffusion method. Methods: 300 strains of Acinetobacter baumannii were randomly selected to detect the distribution of their specimens and their departments. Acinetobacter baumannii was used to determine the effects of Acinetobacter baumannii on CIP, (CN), amikacin (CA), ceftazidime (FEP), levofloxacin (LEV), ampicillin-sulbactam (SAM), tobramycin (MEN), minocycline (MH) and cefoperazone-sulbactam (SCF). The differences between the two test results were compared. Results: Acinetobacter baumannii was mainly distributed in sputum samples, accounting for 71.3% of total, accounting for 71.3%. The main sources were 101 (33.7%) in ICU and 59 (19.7%) in brain surgery. The results of drug sensitivity test showed that the sensitivity of SCF and MH obtained by the two detection methods was statistically significant (P <0.05). There was no significant difference in drug susceptibility between the other 9 antibiotics (P> 0.05). Conclusion: The agar dilution method and disk diffusion method are not completely consistent with the drug susceptibility test results of Acinetobacter baumannii. In clinical use, we should pay special attention to the reliability of drug susceptibility results of SCF and MH.