论文部分内容阅读
目的探讨新疆维吾尔族妇女子宫颈鳞癌血浆中Ras相关区域家族1A(RASSF1A)基因启动子甲基化水平检测的意义,为早期诊断宫颈癌寻找分子标志物。方法采用DNA提取试剂盒提取血浆DNA,甲基化修饰试剂盒使DNA进行亚硫酸氢盐修饰,采用甲基化特异性PCR检测血浆中RASSF1A基因启动子甲基化水平。结果 50例维吾尔族子宫颈鳞癌患者中,8例患者出现RASSF1A基因甲基化,42例未出现RASSF1A基因甲基化,甲基化阳性率为16.00%;45例健康体检妇女检测出1例RASSF1A基因甲基化,44例未出现RASSF1A基因甲基化,甲基化阳性率为2.22%。结论血浆中RASSF1A基因启动子甲基化对寻找维吾尔族妇女子宫颈鳞癌早期诊断分子标志物有重要的指导意义。
Objective To investigate the significance of promoter methylation status of Ras-related region family 1A (RASSF1A) gene in cervical squamous cell carcinoma of Xinjiang Uygur women and find molecular markers for early diagnosis of cervical cancer. Methods Plasma DNA was extracted by DNA extraction kit. Methylated DNA was modified by bisulfite. The promoter methylation of RASSF1A gene in plasma was detected by methylation - specific PCR. Results RASSF1A gene methylation was found in 8 cases of Uigur patients with cervical squamous cell carcinoma, methylation of RASSF1A gene was not found in 42 cases, and methylation was detected in 16 cases, while in 45 cases of healthy women, 1 case was detected RASSF1A gene methylation, 44 cases did not appear RASSF1A gene methylation, methylation-positive rate was 2.22%. Conclusion The promoter methylation of RASSF1A gene in plasma has important guiding significance for the early diagnosis of cervical squamous cell carcinoma in Uigur women.