论文部分内容阅读
采用胸腔注射植物血球凝集素(phytohemagglutinin,PHA)及秋水仙素溶液,取活体头肾细胞经低渗、固定、空气干燥法,分析比较了中华单角鲀(Monacanthus chinensis)、黄鳍东方鲀(Takifuguxanthopterus)、红鳍东方鲀(T.rubripes)的核型。结果表明,3种海水鱼中期染色体均为二倍体,未发现异型性染色体、随体和次缢痕。其核型如下:中华单角鲀的核型为2n=34(34t),臂数:NF=34;黄鳍东方鲀的核型为2n=44(12m+8sm+24t),臂数:NF=64;红鳍东方鲀的核型为2n=44(14m+6sm+24t),臂数:NF=64。中华单角鲀的核型与后两者存在较大差异。同时,将此3种鱼的核型与前人报道的其他鲀形目鱼类核型作了比较。
Thoracic injection of phytohemagglutinin (PHA) and colchicine solution was used to investigate the effects of hypoxia, immobilization and air-drying on the head kidney cells of Monacanthus chinensis, Takifuguxanthopterus), karyotypes of T. rubripes. The results showed that the metaphase chromosomes of three species of marine fish were diploid, and no atypia chromosomes, subscripts and microsatellites were found. Its karyotype is as follows: The karyotype of C. monocytogenes is 2n = 34 (34t), the number of arms is NF = 34; the karyotype of P. orientalis is 2n = 44 (12m + 8sm + 24t) = 64; The karyotype of P. orientalis is 2n = 44 (14m + 6sm + 24t), the number of arms is NF = 64. There is a big difference between the karyotype of C. monkshood and the latter two. At the same time, the karyotypes of these three species of fish were compared with those of other halibut species previously reported.