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目的 :通过舌癌术前化疗增殖细胞核抗原 (PCNA )和细胞凋亡相关基因 bcl- 2、Bax表达的研究 ,探讨舌癌的化疗作用机制。 方法 :对舌乳头状瘤、舌癌和舌癌术前化疗标本共 34例 ,采用免疫组织化学染色技术——酶标链亲和素生物素法 (L SAB)染色并进行光镜下观察。结果:PCNA在舌癌中的表达最强 ,在舌癌术前化疗中的表达明显减弱 (P <0 .0 5 ) ,其与舌乳头状瘤表达相似 (P >0 .0 5 )。 bcl- 2在舌癌术前化疗及舌乳头状瘤中低表达(P >0 .0 5 ) ,舌癌中表达明显增强 (P <0 .0 5 )。Bax在舌癌组和乳头状瘤组中表达有明显差异 (P <0 .0 5 )。结论 :化疗药物抑制肿瘤生长的机制可能与抑制肿瘤细胞增殖和诱导肿瘤细胞程序性死亡有关 ,提示舌癌术前化疗对肿瘤的预后有重要意义。
OBJECTIVE: To investigate the chemotherapeutic mechanism of tongue carcinoma by preoperative chemotherapy of proliferating cell nuclear antigen (PCNA) and expression of apoptosis-related genes bcl-2 and Bax in tongue cancer. Methods: A total of 34 cases of tongue cancer, tongue cancer and tongue cancer were examined by light microscopy using immunohistochemical staining technique and streptavidin-biotinylated streptavidin-biotin (L SAB) staining. Results: The expression of PCNA was the strongest in tongue cancer and significantly decreased in preoperative chemotherapy of tongue cancer (P <0.05). The expression of PCNA was similar to tongue papilloma (P> 0.05). The expression of bcl-2 was significantly lower in tongue cancer preoperative chemotherapy and tongue papilloma (P> 0.05) than in tongue cancer (P <0.05). Bax expression was significantly different between tongue cancer group and papilloma group (P <0.05). Conclusion: The mechanism of chemotherapeutic drugs inhibiting tumor growth may be related to inhibiting the proliferation of tumor cells and inducing apoptosis of tumor cells, suggesting that preoperative chemotherapy of tongue cancer is of great significance for the prognosis of the tumor.