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细胞族是树状突细胞(DC)和淋巴细胞或LAK细胞进行功能活动的微环境,为进一步探讨不同培养时间和培养条件对体外DC形成细胞族功能的影响,本实验将分离的人血DC分别与淋巴细胞和LAK细胞混合培养(37℃,5%CO_2),以DC与4-25个细胞聚集计为一簇,进行活细胞观察计数.结果发现,经16h培养的DC,与淋巴细胞或LAK细胞共孵4h,均形成细胞族(31.50±3.50,20.60±2.15),继续孵育至24h显著增加(50.30±7.10,38.30±5.97,P<0.01),至28h未再出现明显差异(51.20±7.86,40.40±5.18,P>0.1).培养36h的DC与培养16h者相比,与淋巴细胞或LAK细胞共孵4h后细胞族均相应增加(40.10±4.83,33.00±
The cell family is a microenvironment in which dendritic cells (DCs) and lymphocytes or LAK cells perform functional activities. To further explore the effect of different culture time and culture conditions on the cell-forming function of DCs in vitro, the isolated human blood DCs Respectively, and mixed with lymphocytes and LAK cells (37 ℃, 5% CO 2), DC and 4-25 cells aggregated as a cluster, the number of live cells observed and found that after 16h DC cultured with lymphocytes Or LAK cells incubated for 4h, all formed cell populations (31.50 ± 3.50, 20.60 ± 2.15) and continued to increase significantly to 24h after incubation (50.30 ± 7.10, 38.30 ± 5.97, P <0.01) ± 7.86,40.40 ± 5.18, P> 0.1). Compared with those cultured for 16h, DCs cultured for 36h showed a corresponding increase in cell population after incubation with lymphocytes or LAK cells for 4h (40.10 ± 4.83 and 33.00 ±